基于N蛋白的PDCoV抗体间接ELISA检测方法的建立和应用
Yuting Xiao1, Lei Zhou1, Qin Gao1
1Department of Veterinary Medicine, College of Animal Sciences, Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310058, China.
Veterinary sciences
|January 28, 2026
概括
一个新的ELISA测试使用重组N蛋白检测猪三角型冠状病毒 (PDCoV) 抗体. 这种敏感和特定的测试有助于PDCoV监测和猪病管理.
科学领域:
- 兽医病毒学 兽医病毒学
- 免疫诊断 免疫诊断 免疫诊断 免疫诊断
背景情况:
- 猪三角型冠状病毒 (PDCoV) 对全球猪业构成重大威胁.
- 准确的诊断工具对于PDCoV监测和控制至关重要.
研究的目的:
- 开发和验证一种敏感和特定的酶相关免疫吸收试验 (ELISA) 用于检测PDCoV抗体.
- 评估江省猪群中PDCoV抗体的流行情况.
主要方法:
- 使用baculovirus系统表达的重组核囊体 (N) 蛋白被用作抗原.
- 该ELISA测定被验证为敏感性,特异性和与其他猪病毒 (TGEV,PEDV) 的交叉反应性.
- 开发的ELISA被用于测试来自江省的600个猪血清样本 (2021-2024年).
主要成果:
- 艾丽莎测试显示高灵敏度 (96.67%) 和特异性 (85.51%) 具有良好的可重复性 (CV < 10%).
- 没有观察到与传染性胃肠炎病毒 (TGEV) 或猪流行性腹病毒 (PEDV) 的交叉反应.
- 在江省的流行分析显示了PDCoV抗体水平的显著区域差异和时间波动.
结论:
- 成功开发了一种敏感和特定的ELISA方法来检测抗PDCoV N抗体.
- 这种测定作为PDCoV监测,流行病学调查和猪病管理的可靠诊断工具.
- 这些发现强调了在猪群中持续监测PDCoV的重要性.
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