颜色检测RT-LAMP测试用于快速,灵敏和特定检测猪流产相关虫害病毒 (PAAPeV)
Xu Yang1,2, Ying Li3, Wenqi Yin1,2
1Animal Genetic Breeding and Reproduction Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, China.
开发了一种新的色度测定方法,用于检测猪流产相关的农虫病毒 (PAAPeV). 这种快速,灵敏和特定的工具有助于诊断猪的生殖障碍,为现场和实验室设置提供了有价值的诊断选择.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子诊断学 分子诊断
- 异热放大技术 异热放大技术
背景情况:
- 猪流产相关杀虫病毒 (PAAPeV) 是一种新兴的病原体,在猪中引起严重的生殖问题.
- 目前缺乏有效的治疗方法或疫苗,需要改进PAAPeV的诊断工具.
研究的目的:
- 开发和优化用于PAAPeV检测的色度反转录循环介导同热放大 (RT-LAMP) 试验.
- 为了验证测试的特异性,灵敏度和诊断性能与既有方法相比.
主要方法:
- 设计和选针对PAAPeV 5'未翻译区域 (5'UTR) 的四组LAMP开头.
- 针对色度测定RT-LAMP试验的反应温度,持续时间和原料比率的优化.
- 验证特异性和敏感性,与逆转录定量聚合酶链反应 (RT-qPCR) 相比.
主要成果:
- 优化的试验实现了2个副本/μL的检测极限,结果可用肉眼进行解释.
- 该试验显示高特异性,没有对其他农药病毒或猪病原体的交叉反应.
- 在临床样本测试中,在colordetect RT-LAMP测定和RT-qPCR测定之间观察到100%的一致性.
结论:
- 开发的色度测定RT-LAMP试验是一种快速,灵敏和特定的PAAPeVRNA检测工具.
- 这种检测方法适用于临床实验室和现场环境,用于诊断PAAPeV感染.
- 该试验为管理由PAAPeV引起的猪生殖障碍提供了有价值的诊断解决方案.
更多相关视频
06:57Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens
Published on: February 20, 2015
08:03Reverse Transcription-Loop-mediated Isothermal Amplification RT-LAMP Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
Published on: September 14, 2018
相关概念视频
Sensitivity, Specificity, and Predicted Value
Sensitivity is the...
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Specific Heat
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or...
Sputum Studies II: Culture and Sensitivity
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Rapidly Varying Flow
Density, Specific Weight, Specific Gravity and Compressibility of Fluid
Specific weight represents the weight per unit volume and is calculated by multiplying...
