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基于LAMP的快速检测,在野外种植的番茄植物中混合贝戈莫病毒感染

Yoslaine Ruiz-Otaño¹, Berenice Calderón-Pérez², Rosabel Pérez Castillo¹

¹Departamento de Biotecnología de las Plantas, Centro de Ingeniería Genética y Biotecnología, Av. 31e/158 y 190, Playa, Apdo, 6162, Habana 10600, Cuba.

|January 28, 2026

概括

在作物中早期检测贝戈莫病毒至关重要. 一种灵敏的循环介导同热放大 (LAMP) 试验有效地在植物中识别出番茄病毒,即使是无症状的植物,性能也优于传统方法.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
农业科学农业科学

背景情况:

植物病原性病毒,特别是贝戈莫病毒,在全球范围内造成大量的作物损失.
病毒性疾病的有效管理依赖于在作物和杂草中早期和准确地检测病原体.
亚热带和热带地区特别容易受到begomovirus的威胁.

研究的目的:

评估一种敏感的循环介导同热放大 (LAMP) 试验,用于检测关键的贝戈莫病毒.
评估测试在识别番茄黄色叶子卷曲病毒 (TYLCV),番茄潜伏病毒 (TLV) 和番茄斑点泰诺病毒 (ToMoTV) 的有效性.
在现场条件下比较LAMP测试性能与点点杂交和PCR.

主要方法:

循环介导同热放大 (LAMP) 试验的应用.
在农业注射 *Nicotiana benthamiana *和 *Solanum lycopersicum * (番茄) 上进行测试.
在症状和无症状的田间种植的番茄植物中检测.

主要成果:

在实验和野外种植的植物中,LAMP试验成功检测出TYLCV,TLV和ToMoTV.
LAMP发现的受感染植物数量超过了点点杂交和PCR.
实地调查显示,在单个番茄植物内,这些病毒的复杂混合感染.

结论:

开发的LAMP测试是一种敏感且有效的工具,用于在西红中早期检测贝戈莫病毒.
这种方法有助于理解涉及多种病毒感染的复杂流行病学场景.
准确的检测有助于及时制定管理策略,以减轻贝戈莫病毒造成的作物损失.

关键词:

一个灯的灯光.农业感染农业感染开始了病毒病毒.混合感染混合感染番茄潜伏病毒病毒番茄斑点泰诺病毒泰诺病毒番茄黄色叶子卷曲病毒病毒

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