细胞表面的维门是一种附着因子,可以促进马关节炎病毒的体外感染
Côme J Thieulent1, Sanjay Sarkar2, Mariano Carossino1,3
1Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Viruses
|January 28, 2026
概括
马类动脉炎病毒 (EAV) 使用维门丁作为感染的附着因子,扩大了我们对其广泛宿主细胞热流的理解,超出了之前识别的EqCXCL16S受体.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 蛋白相互作用 蛋白相互作用
背景情况:
- 马类动脉炎病毒 (EAV) 呈现广泛的宿主细胞热带性,感染的细胞超出表达已知的受体,马类CXCL16 (EqCXCL16S) 的细胞.
- 这表明EAV利用额外的宿主细胞蛋白质来促进感染,并解释其多样化的细胞点.
研究的目的:
- 识别与EAV相互作用并导致其感染的其他宿主细胞蛋白质.
- 调查维门丁作为潜在的EAV进入因子的作用.
主要方法:
- 病毒覆盖蛋白结合试验和远西部斑被用来识别马细胞中的EAV结合蛋白.
- 液体染色学-双重质谱法 (LC-MS/MS) 用于蛋白质的鉴定.
- 维门丁的作用通过细胞系查,抗体中和基因过度表达研究来评估.
主要成果:
- 一种57kDa的膜蛋白被确定为潜在的EAV结合蛋白,随后通过LC-MS/MS被确定为维门丁.
- 在各种哺乳动物细胞系中,EAV感染易感性与维门丁表达相关.
- 抗维门丁抗体和Withaferin A部分抑制了EAV感染,而维门丁过度表达增强了它.
结论:
- 马类维门作为EAV的附着因子,积极参与感染过程.
- 通过使用多种宿主细胞蛋白质,包括维门丁,以实现其广泛的细胞热带性.
- 这一发现为EAV与宿主相互作用和病毒进入机制提供了新的见解.
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