工程AQP1-缺陷的DF-1悬浮细胞用于高产量IBDV生产和疫苗扩展
Bingmei Dong1, Ruonan Wang2, Yu Guan2
1College of Life Science, Cangzhou Normal University, Guofeng South Avenue 16#, Cangzhou 061001, China.
Vaccines
|January 28, 2026
概括
在DF-1细胞中破坏水素-1 (AQP1) 形成了一个稳定的悬浮细胞系. 这种工程化鸟类细胞系显著增强了用于疫苗制造的传染病病毒 (IBDV) 生产.
科学领域:
- 细胞生物学 细胞生物学
- 病毒学 病毒学
- 生物技术是生物技术.
背景情况:
- 大规模的家禽病毒疫苗生产需要可扩展的细胞平台.
- 大多数鸟类细胞系,如DF-1,都依赖于位,限制了可扩展性.
- 水素-1 (AQP1) 影响细胞粘附和膜动力学,成为悬浮生长工程的目标.
研究的目的:
- 通过破坏Aquaporin-1 (AQP1) 基因来产生稳定的DF-1悬浮细胞系.
- 评估工程细胞系对增强传染性突发性疾病病毒 (IBDV) 生产的潜力.
主要方法:
- 使用CRISPR/Cas9设计的DF-1细胞破坏了AQP1基因,从而产生了DF-1/AQP1−细胞.
- 评估了细胞形态,瘤性,遗传稳定性和悬浮生长特征.
- 在悬浮DF-1/AQP1−细胞,单层DF-1/AQP1−细胞和野生型DF-1细胞之间比较IBDV生产标位.
主要成果:
- DF-1/AQP1−细胞表现出正常的形态,非瘤性,并保持稳定的AQP1突变.
- 实现了真正的悬浮生长,具有高细胞密度 (4.0 × 106 细胞/毫升) 和活力 (> 95%).
- 悬浮的DF-1/AQP1−细胞产生了显著更高的IBDV标位 (9.0 log TCID50/mL;8.63 log EID50/mL) 和减少了生产时间.
结论:
- 有针对性的AQP1干扰成功地产生了稳定,非瘤源的DF-1悬浮细胞系.
- 工程细胞系显示出明显增强的IBDV生产能力.
- 这为下一代禽疫苗制造提供了一个可扩展的平台.
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