相关实验视频
Updated: Jan 29, 2026

Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
暴露于甲诱导面骨发育毒性通过ROS诱导的氧化应激和斑马鱼 (Danio rerio) 胚胎的亡
Runhao Zhu1, Yong Huang2, Xinyue Chang3
1Department of Cariology & Endodontics, Affiliated Stomatological Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330006, China; Jiangxi Branch of State Key Laboratory for Macromolecule Drugs and Large-scale Preparation & National Engineering Research Center of Cell Growth Factor Drugs and Protein Biologics, Center for Genetic Development and Regenerative Medicine, First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi Province, China; Jiangxi Key Laboratory of Oral Biomedicine, Jiangxi Clinical Medical Research Center of Oral Diseases, Nanchang, Jiangxi 330006, China.
甲 (PrP) 暴露会通过诱导软骨细胞的氧化应激和亡,导致斑马鱼的面形. 抗氧化剂阿斯塔克桑部分扭转了这些有害影响.
科学领域:
- 毒理学 毒理学 毒理学
- 发展生物学 发展生物学
- 生物化学 生化学
背景情况:
- 甲 (PrP) 是一种广泛使用的防腐剂.
- 它对动物发育的毒理影响尚未完全理解.
研究的目的:
- 调查PrP对斑马鱼胚胎和幼虫的毒理影响.
- 阐明 PrP 诱导的发育异常背后的机制.
主要方法:
- 斑马鱼胚胎暴露于不同度的PrP.
- 分析了面发育,氧化应激标志物,基因表达和亡.
- 阿斯塔克桑丁被用作联合治疗,以评估其保护作用.
主要成果:
- 暴露PrP导致了显著的面软骨形变.
- 观察到氧化应激标志物 (SOD,CAT,MDA) 的升高.
- 亡增加了面冠状细胞,并降低了冠状基因表达的调节.
结论:
- PrP诱导的氧化应激会导致冠状细胞亡和面形.
- 阿斯塔克桑丁证明了PrP诱导的发育毒性的部分救援.
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