在Xenopus神经发育过程中,内源ADIP的平面极化
Satheeja Santhi Velayudhan1, Keiji Itoh1, Chih-Wen Chu1
1Department of Stem Cell Biology and Regenerative Medicine, Icahn School of Medicine at Mount Sinai, New York, 10029, USA.
Biology open
|January 28, 2026
概括
在组织修复和神经管关闭过程中,阿法丁和α-actinin结合蛋白 (ADIP) 重组,表明它感知机械线索. 细胞骨网络调节了这种机械敏感蛋白质.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 协调的细胞极性和强力响应蛋白质定位对于组织形态发生是至关重要的.
- 了解胚胎细胞如何感知和响应机械线索是一个关键的挑战.
- 亚法丁和α-actinin结合蛋白 (ADIP) 涉及微管体动力学,纤维生成和集体细胞迁移.
研究的目的:
- 研究早期脊椎动物胚胎内源性ADIP的分布和调节.
- 为了确定ADIP是否在体内充当机械敏感蛋白的功能.
- 阐明细胞骨架网络在ADIP两极化中的作用.
主要方法:
- 在上皮层伤口修复和神经管关闭期间观察Xenopus胚胎内源性ADIP局部化.
- 平面细胞极性 (PCP) 组成部分的耗尽 Diversin/Ankrd6.6.
- 微管,F-actin和非肌肉肌肉蛋白II的药理学破坏.
主要成果:
- 内生ADIP在上皮层伤口修复过程中表现出动态重组和两极分化.
- 在前神经板中,ADIP变得丰富且平面极化,在神经管关闭过程中与机械力相关.
- ADIP的两极分化取决于核心PCP成分Diversin/Ankrd6和细胞骨元素 (微管,F-actin,非肌肉肌肉蛋白II).
结论:
- 内生ADIP作为机械敏感蛋白,通过细胞骨机械感知机械线索.
- 在脊椎动物形态发生过程中,ADIP在调节集体细胞行为方面发挥着上下文依赖的作用.
- 微管和actomyosin网络对于神经外皮中的ADIP两极分化至关重要.
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