亚临床感染与手术诱导的认知功能障碍相结合:一个新型的成年老鼠模型,用于外科手术期间的神经认知障碍
Chenchen Xia1,2, Xiao Zhang1,2, Wanbing Dai1,2
1Department of Anesthesiology, School of Medicine, Renji Hospital, Shanghai Jiaotong University, Shanghai, China.
Frontiers in aging neuroscience
|January 28, 2026
概括
手术前的感染使免疫系统敏感,导致手术后的神经炎症和认知缺陷. 这项研究通过将低剂量脂多糖 (LPS) 与手术相结合,开发了一种治疗术前神经认知障碍 (PND) 的小鼠模型.
科学领域:
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
- 麻醉学 麻醉学
背景情况:
- 外科手术周围神经认知障碍 (PND) 是手术和麻醉后的一个重大问题,通常与神经炎症有关.
- 现有的模型没有完全捕捉到可能导致PND的手术前的亚临床炎症状态.
研究的目的:
- 建立和验证一种新的成年老鼠模型,用于PND.
- 研究手术前的亚临床感染与手术对认知功能和神经炎症的影响.
主要方法:
- 成年雄性小鼠在手术前 (骨骨折固定或尾切除) 接受低剂量脂聚糖 (LPS).
- 行为测试 (开放场地,新型物体识别,莫里斯水迷宫) 评估了活动,焦虑和认知.
- 使用ELISA,RT-qPCR,西斑和蛋白质组学分析了海马炎症标记物,突触蛋白和分子通路.
主要成果:
- 手术前的LPS诱导了手术后的神经炎症和微质激活的增加.
- 小鼠在学习和记忆方面表现出显著的障碍.
- 关键突触蛋白 (PSD-95,GAP-43,SYN,BDNF) 的降低水平表明突触功能受损.
- 蛋白质组学揭示了免疫反应,突触组织和能量代谢的改变途径.
结论:
- 成功开发了一种用于PND的实用成年老鼠模型.
- 该模型表明,手术前的炎症与手术相结合会引发神经炎症和认知缺陷.
- 这个模型提供了对PND背后的分子机制的见解.
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