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Updated: Jan 29, 2026

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洞察阿特佐利祖马布在多重应力下与非极性氨基酸基离子液体的合体和结构稳定性:第二阶段
Bayan Alkhawaja1,2, Faisal Al-Akayleh1, Saifeddin Daadoue1
1Faculty of Pharmacy and Medical Sciences, University of Petra Amman 11196 Jordan bayan.alkhawaja@uop.edu.jo ba436@bath.ac.UK.
RSC advances
|January 28, 2026
概括
可再生胆酸盐 (CV) 离子液体显著增强单克隆抗体 (mAb) 对热和化学压力的稳定性. 与其他配方相比,CV提供了优越的保护,改善了治疗保质期.
科学领域:
- 生物制药配方 生物制药配方
- 蛋白质化学 蛋白质化学
- 绿色化学 绿色化学
背景情况:
- 单克隆抗体 (mAbs) 面临稳定性挑战,影响治疗疗效和保质期.
- 物理和化学不稳定性是mAb配方中的关键问题.
- 开发稳定的mAb配方对于生物制药开发至关重要.
研究的目的:
- 调查基于胆氨基酸的离子液体 (Ch-AA ILs),特别是胆瓦林酸 (CV) 和胆甘酸 (CG),对单克隆抗体 (mAbs) 的稳定潜力.
- 评估CV和CG在各种压力条件下增强阿特佐利祖马布 (Amab) 的体和结构稳定性的有效性.
主要方法:
- 亚特佐利祖马布 (Amab) 的配方,含有不同度的胆瓦林酸 (CV) 和胆甘酸 (CG).
- 将配方置于环境,热 (40-70°C) 和化学 (尿素) 压力之下.
- 使用动态光散射 (DLS),紫外线可见光谱 (聚合指数) 和ATR-FTIR (二次结构分析) 评估mAb的稳定性.
主要成果:
- 胆瓦林酸 (CV),特别是在30%w/w时,与胆甘酸 (CG) 和Tris-HCl缓冲剂相比,Amab的体稳定性优越.
- 在严重的热应激下 (24小时70°C),30%的CV配方显示出最小的聚合 (AI ≤17),而Tris缓冲器中的Amab则大量聚合 (AI >160).
- 由于离子液体引起的细胞代谢活动的非特异性增加,MTT对生物活性的测定结果是不确定的.
结论:
- 胆酸 (CV) 显示出作为单克隆抗体 (mAbs) 的稳定辅助剂的显著前景.
- 可再生溶剂CV增强了mAbs的体和结构稳定性,为生物制药配方提供了绿色替代品.
- 需要进行进一步的研究,以使用与离子液体配方相容的适当测试来验证生物活性.
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