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采集心脏穿刺血液作为一种实用和生物安全的方法,用于死后检测猪的病原体
Claudio Marcello Melini1, Mariana Kikuti1, Xiaomei Yue1
1Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN, United States.
Frontiers in veterinary science
|January 28, 2026
概括
心脏穿刺 (CP) 提供了一种有效的方法来收集死猪的血液样本. 这种技术有助于检测猪病毒,如古典猪瘟和非洲猪瘟,而不会污染环境.
科学领域:
- 兽医病理学 兽医病理学
- 传染病诊断 传染病诊断 传染病诊断
- 动物病毒学 动物病毒学
背景情况:
- 活猪样本采集通常是劳动密集型,可能需要身体克制.
- 现有的对活体动物的非侵入性方法在应用或病原体检测方面存在局限性.
- 对死动物的替代方法可能导致血液泄漏,对猪病原体构成生物封闭风险.
研究的目的:
- 评估心脏穿刺 (CP) 作为收集死猪血液样本的可行替代方案.
- 评估CP用于检测猪病毒的实用性,特别是在古典猪瘟和非洲猪瘟的背景下.
- 为了确定CP是否可以减轻样本采集期间与环境污染相关的风险.
主要方法:
- 在两项研究中,在病毒爆发期间采用心脏穿孔来收集来自不同年龄的安乐死或死亡猪的血液.
- 来自286只猪的血液样本被采集,其中273只猪提供了足够的血清供分析.
- 在收集的血清样本上进行了猪生殖和呼吸系统病毒检测.
主要成果:
- 通过心脏穿刺,成功从286头猪收集了血液.
- 在273个测试样本中,95%的样本对目标病毒呈阳性检测.
- 该方法被证明可用于获取血液和测试血清,产生高检测率.
结论:
- 心脏穿刺是一种有效和可行的方法,用于从死猪中获取血样,用于病毒诊断.
- 这种技术尽量减少与流血有关的环境污染风险.
- 在猪病爆发调查期间,CP为采集样本提供了有价值的替代工具,特别是对于高后果病原体.
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