血小板衍生的脊椎生物学药物的中期储存:一种可行的替代品,用于马类实践
Sarah Raphaela Torquato Seidel1, Joice Fülber2, Ângela Perrone Barbosa1
1Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
Frontiers in veterinary science
|January 28, 2026
概括
储存富含血小板血 (PRP) 衍生物,如血小板溶解盐 (PL) 和冷解PRP (FD-PRP),可以延长保质期. 这些加工的脊椎生物学物长时间保持治疗生长因子含量,改善马类兽医应用.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 整形外科 整形外科 整形外科
- 生物技术是生物技术.
背景情况:
- 富血小板血 (PRP) 是一种常见的马体生理病.
- 目前的PRP协议需要新的准备,限制了可用性.
- 需要可存储的PRP衍生产品.
研究的目的:
- 为了开发可储存的PRP衍生的骨科生物.
- 在延长储存后评估生长因子含量.
- 为了保持PRP衍生物的治疗疗效.
主要方法:
- 准备的PRP,血小板溶解盐 (PL) 和冷解PRP (FD-PRP).
- 在 -80°C, -20°C和室温 (RT) 中保存产品30天.
- 评估长期储存 (1-2年) 在-80°C和-20°C,以及FD-PRP的RT.
主要成果:
- 在30天后,在-80°C时没有显著的TGF-β1差异.
- 在-20°C时,PL保持高TGF-β1的水平.
- 与PL相比,RT储存减少了炎症性细胞因子 (IL-1β,IL-10,TNF-α),但在PRP和FD-PRP中增加了TGF-β1.
结论:
- 开发了生产可储存的PRP衍生物 (PL,FD-PRP) 的方法.
- 这些产品在制备量和储存方面提供了灵活性.
- 允许在兽医设施进行现场储存,以便立即使用.
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