超越传统的转基因生物:一种全面的方法来识别未知的Cisgenic大米事件
Shengtao Lu1, Jiajian Xie2, Fan Wang1
1Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Hainan Research Institute, Shanghai Jiao Tong University, Shanghai, 200240, China.
The New phytologist
|January 28, 2026
概括
未经授权的cisgenic大米,是为了耐受草甘而设计的,被发现逃避检测. 目前的监测系统需要升级为基因组方法,以实现有效的生物安全和贸易监管.
科学领域:
- 农业生物技术 农业生物技术
- 植物遗传学 植物遗传学
- 生物安全法规 生物安全法规
背景情况:
- 未经授权的耐除除草剂的米品种对监管系统构成了挑战.
- 农作物中的表型异常需要分子调查以确定遗传修饰.
研究的目的:
- 为了分子表征未经授权的耐草甘米.
- 开发针对 cisgenic 事件的敏感检测方法.
- 评估当前转基因生物 (GMO) 监测的有效性.
主要方法:
- 综合分子分析包括PCR,qPCR,Illumina全基因组测序和PacBio长读测序.
- 生物信息分析用于识别整合场所和插入结构.
- 开发基因特异性和事件特异性检测试验.
主要成果:
- 确定了六个独立的cisgenic大米事件,其中具有突变的OsEPSPS基因,赋予了草甘耐受性.
- 每个事件都显示出独特的染色体整合部位和复杂的并联重复结构.
- 检测试验符合国际标准,检测极限为0.05-0.1%.
结论:
- 目前的转基因生物监测系统不足以检测转基因产品.
- 未经授权的cisgenic作物可以绕过传统的监管监督.
- 转向全面的基因组检测方法对于生物安全和贸易至关重要.
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