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相关概念视频

Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

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Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
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Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.4K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

10.6K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

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The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
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相关实验视频

Updated: Jan 29, 2026

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
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Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

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非向的shRNA编码的等离子体DNA通过寨卡病毒动物模型的RIDD-RIG-I信号通路增强了保护性免疫力.

Min-Syuan Huang1,2, Hung-Chun Liao1, Po Peng3

  • 1National Institute of Infectious Disease and Vaccinology, National Health Research Institutes, Miaoli, Taiwan.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|January 28, 2026
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种针对寨卡病毒 (ZIKV) 的新型DNA疫苗,该疫苗使用短毛RNA (shRNA) 作为辅助剂来增强免疫反应. 增强的DNA疫苗在临床前模型中表现出更好的疗效,为传染病预防提供了一个有前途的平台.

关键词:
寨卡病毒 寨卡病毒免疫性 免疫性 免疫性分子辅助剂 分子辅助剂不针对性的shRNA.调节的IRE1依赖衰变 (RIDD) 路径.

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Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
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Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

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Generation of Recombinant Influenza Virus from Plasmid DNA
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Generation of Recombinant Influenza Virus from Plasmid DNA

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Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
07:53

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Published on: January 18, 2019

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Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
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Generation of Recombinant Influenza Virus from Plasmid DNA
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Generation of Recombinant Influenza Virus from Plasmid DNA

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科学领域:

  • 疫苗学 疫苗学 疫苗学
  • 分子生物学分子生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • DNA 疫苗为传染病控制提供了具有成本效益和稳定的平台.
  • 由于DNA疫苗的免疫性有限,因此需要制定增强免疫反应的策略.
  • 寨卡病毒 (ZIKV) 仍然是一个重大的公共卫生问题,需要有效的疫苗解决方案.

研究的目的:

  • 用一种新型的内在分子辅助剂开发一种针对ZIKV的增强型DNA疫苗.
  • 研究shRNA增强先天免疫信号传递和疫苗免疫性机制.
  • 在临床前的小鼠模型中评估开发的DNA疫苗的疗效.

主要方法:

  • 开发一种编码ZIKV prM-E蛋白质的DNA疫苗,其中包含一个非向的shRNA.
  • 研究IRE1α通路和RIG-I信号的shRNA介导激活.
  • 在小鼠模型中评估疫苗诱导的幽默和细胞免疫力,中和抗体标位,病毒血症和生存率.

主要成果:

  • 使用shRNA辅助剂的DNA疫苗引起了强大的ZIKV特异性幽默和细胞免疫反应.
  • 在接种疫苗的AGB6小鼠中观察到中和抗体标位的显著增加和更好的生存率.
  • 通过RIG-I激活,减少病毒血症和增强先天性免疫信号被证实.

结论:

  • 开发的DNA疫苗有效地增强了免疫性,而不准宿主基因,利用shRNA作为内在辅助剂.
  • 这种方法为开发下一代抗传染病DNA疫苗提供了一个可扩展和适应的平台.
  • 这些发现支持基于shRNA的辅助剂在改善DNA疫苗疗效方面的潜力.