在环境样本中使用功能性元基因组学识别的Cefiderocol耐药基因
Remi Gschwind1, Mehdi Bonnet1,2, Anna Abramova3,4,5
1Université Paris Cité, INSERM, Université Sorbonne Paris Nord, IAME, F-75018 Paris, France.
The ISME journal
|January 28, 2026
概括
在使用功能元基因组学的环境样本中发现了新的 cefiderocol 耐药基因. 这一发现对于改善抗生素耐药性监测和了解环境中耐药性基因的传播至关重要.
科学领域:
- 环境微生物学环境微生物学
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 抗生素耐药性是一种全球性健康威胁,由环境基因转移加剧.
- 最后一线抗生素Cefiderocol面临着新兴的耐药性挑战.
- 现有的数据库缺乏对 cefiderocol 耐药性机制的描述.
研究的目的:
- 在环境样本中识别和表征新的 cefiderocol 耐药基因.
- 评估这些基因的流行率和传播潜力.
- 改进抗生素耐药性的监测策略.
主要方法:
- 功能性元基因组学应用于各种环境样本 (废水,淡水,土壤).
- 鉴定赋予塞菲德罗科尔最低抑制度增加的基因.
- 生物信息分析以确定基因同类和移动遗传元素的关联.
主要成果:
- 发现了四种新的 cefiderocol 耐药基因:三种β-乳糖酶 (VEB-3,OXA-372同源,YbxI同源) 和一种青素结合蛋白同源.
- 这些基因增加了对临床相关水平 (1-4 mg/L) 的 cefiderocol 耐药性.
- 三个基因在致病性细菌中具有同类; blaVEB-3 在废水中广泛存在,并与移动遗传元素相关.
结论:
- 功能性转基因组学对于发现未表征的抗生素耐药性机制至关重要.
- 在环境中存在新的抗塞菲德罗科尔基因,需要改进监测.
- 鉴定这些基因将增强抗生素耐药性基因数据库,并支持"一个健康"倡议.
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