开发一种基于阻抗的快速抗微生物敏感性测试,以检测Neisseria gonorrhoeae
Caitlin N Daniels1, Bethany K Martin1, Kazi S Nahar2
1Antimicrobial Discovery, Development and Diagnostics, Countermeasures Development, Evaluation and Preparedness Division, UK Health Security Agency, Porton Down, Salisbury, Wiltshire SP4 0JG, UK.
一个新的基于阻抗的测试 (iFAST®) 在不到8小时内为尼塞利亚淋病提供了快速的抗菌感应性测试. 这种快速,准确的方法有助于打击抗菌素耐药性,并优化抗生素治疗.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 临床诊断 临床诊断 临床诊断
背景情况:
- 尼塞利亚淋病菌中的抗菌素耐药性构成了全球健康的重大威胁.
- 目前的抗微生物敏感性测试 (AST) 方法耗时 (≥2天),导致广泛的抗生素过度使用.
- 快速诊断对于有效治疗和打击耐药性至关重要.
研究的目的:
- 开发和评估一种快速的,基于阻抗的,针对尼塞利亚淋病的表型AST.
- 评估iFAST®系统与前线和实验性抗生素的性能.
- 为传统的AST方法提供更快的替代方案.
主要方法:
- 使用了WHO尼塞利亚淋病菌的参考菌株,在断点度和连续稀释时暴露于抗生素.
- 采用基于阻抗的快速抗微生物敏感性测试 (iFAST®) 来测量细胞计数的变化%.
- 将iFAST®结果与已公布的最小抑制度 (MIC) 和新型化合物的Etest值进行比较.
主要成果:
- 在4小时内,iFAST® 显示出与已公布数据一致的敏感性概况.
- 由iFAST®生成的剂量反应曲线与已发表的Etest MIC值在很大程度上一致.
- 新型化合物与西普洛克萨相比,对抗性菌株具有增强的抗菌活性.
结论:
- 该iFAST®方法提供快速 (8小时以下) 和准确的表型AST用于Neisseria gonorrhoeae.
- 结果与当前的临床标准一致,为缓慢的方法提供了一个有希望的替代方案.
- 这项技术可以支持优化抗生素选择和打击抗菌素耐药性.
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