在模拟微重力条件下对C2C12细胞和小鼠血清中N-甘氨酸变异的相关性分析,使用基于四级化标签策略的质谱量测量方法
Qian-Ru Ma1, Jie-Cheng Lu1, Yan-Qing Li1
1Department of Chemistry, Department of Chemical Biology, Fujian Key Laboratory of Chemical Biology (Xiamen University), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, 361005, P.R. China.
Analytical and bioanalytical chemistry
|January 28, 2026
概括
我们开发了一种具有成本效益的标签试剂,用于使用质谱测量进行N-glycan分析. 这种方法可以识别早期肌肉缩诊断的潜在生物标志物.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 生物标志物发现发现
背景情况:
- 具有稳定同位素标记的质谱测量对于通过糖化样式分析识别疾病生物标志物至关重要.
- 现有的稳定同位素标记试剂缺乏灵敏度,负担能力和稳定的酸修饰能力.
研究的目的:
- 开发一种方便,具有成本效益的微波辅助方法,用于合成稳定的同位素标记试剂对 (14N/15N-P4HZD).
- 为了使用HPLC-ESI-HRMS进行敏感和方便的N-甘氨酸的定量差异分析.
- 为了确定肌肉缩的潜在N-甘氨酸生物标志物.
主要方法:
- 14N/15N-P4HZD稳定同位素标记试剂对的微波辅助合成.
- 使用高性能液体染色学-电子喷雾电离-高分辨率质谱法 (HPLC-ESI-HRMS) 分析N-甘氨酸的定量差异分析.
- 该方法用于分析C2C12细胞和后肢中的N-葡萄糖特征,用于卸载肌肉缩的小鼠血清模型.
主要成果:
- 开发的方法证明了高标签效率,可重复性和线性 (R2 = 0.9984) 在两个数量级上.
- 14N/15N-P4HZD试剂提高了电离效率和检测化N-糖的性能.
- N-甘氨酸Man3GlcNAc3Fuc1和Man3GlcNAc4Gal1Fuc1Sia1被确定为肌肉缩的潜在早期诊断生物标志物.
结论:
- 14N/15N-P4HZD试剂对为N-甘定量差异分析提供了一个方便而敏感的策略.
- 这种基于质谱的方法显示了发现临床生物标志物的巨大潜力,特别是肌肉缩.
- 已识别的N-甘氨酸可以作为早期肌肉缩诊断的有价值的生物标志物.
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