从阿萨姆邦出售的动物来源食品中分离出产黄金葡萄球菌和MRSA的肠毒素和生物膜
Vishnu Raghavendran Av1, Zunjar Baburao Dubal2,3, Vinodh Kumar O R1
1Division of Veterinary Public Health, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, Uttar Pradesh, India.
概括
在动物来源的食品中发现的多抗药性金黄色葡萄球菌菌株,可以产生毒素并形成生物膜. 这项研究确定了来自生牛奶和肉类的金黄色菌中的毒素和生物膜基因,突出了食品安全方面的担忧.
科学领域:
- 食品微生物学 食品微生物学
- 抗微生物耐药性 抗微生物耐药性
- 细菌病原体的产生
背景情况:
- 来自动物的食品 (ADF) 可能含有产生毒素的多药耐药 (MDR) 黄金葡萄球菌,构成全球重大食品安全风险.
- 黄金葡萄球菌菌株令人担忧,因为它们有可能产生毒素并形成生物膜,这可能导致食源性疾病和持久性感染.
研究的目的:
- 评估在印度阿萨姆市销售的动物来源食品中获得的黄金葡萄球菌分离物的毒素和生物膜生产能力.
- 为了确定特定毒素基因 (pvl, sea, sed, seb, sei, tst) 和生物膜相关基因 (icaA, icaB, icaC, icaD, clfA, clfB, fnbA) 在S. aureus分离物中的流行率.
主要方法:
- 从138个动物来源的食品样本中分离和识别葡萄球菌种类.
- 抗微生物敏感性测试和mcA基因的检测,以识别多药耐药性和甲素耐药性黄金色细菌 (MRSA).
- 聚合酶链反应 (PCR) 用于毒素类型和检测生物膜相关基因.
主要成果:
- 在138个分离物中,有29个 (21.01%) 被确定为金黄色细菌,主要来自原奶. 观察到多药耐药性 (82.75%) 和甲素耐药性 (27.58%) 的高患病率.
- 检测到的关键毒素基因包括pvl (20.69%),海洋 (37.93%) 和sed (17%). 经常发现与生物膜相关的基因,如icaA (65.51%) 和clfA (51.72%),其中72.41%的分离物具有至少一个生物膜基因.
- 在不同动物来源的食物来源中,在某些毒素和生物膜基因的分布上发现了显著的差异 (P < 0.05).
结论:
- 印度阿萨姆邦的动物来源食品被携带毒性基因的多药耐药黄金葡萄球菌菌株污染.
- 在这些分离物中存在毒素和生物膜基因,表明存在大量的食物传播疾病风险和食品安全管理方面的挑战.
- 有效的监测和控制策略至关重要,以减轻食物链中这些耐药和有毒的金黄色细菌菌株所带来的公共卫生威胁.
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