相关实验视频
Updated: Jan 30, 2026

08:22
Studying Copper Nanoparticle-Induced Programmed Cell Death in Bacteria
Published on: May 16, 2025
580
从克西兰酶固定在聚氨酸表面上的果汁澄清的丰富,使用氨酸覆盖的铜纳米颗粒
Shivam Sharma1, Sudarshan Sahu1, Shailendra Kumar Arya1
1Department of Biotechnology Engineering, University Institute of Engineering and Technology, Panjab University, Chandigarh, India.
Preparative biochemistry & biotechnology
|January 28, 2026
概括
研究人员开发了一种简单的方法,使用铜纳米粒子在聚烯上固定酶. 这种技术增强了酶的稳定性,提高了果汁的清晰度,显示出对工业应用的希望.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 酶工程是什么? 酶工程是什么?
背景情况:
- 酶固定对于工业应用至关重要,增强稳定性和可重复使用性.
- 开发高效和成本效益的固定化技术仍然是一个关键的挑战.
- 聚烯表面为材料功能化提供了一个多功能平台.
研究的目的:
- 在聚烯表面开发一种简单有效的酶固定方法.
- 利用囊功能化铜纳米颗粒作为酶定的桥梁剂.
- 为了评估固定化西兰酶在果汁澄清中的性能.
主要方法:
- 通过用 (3-mercaptopropyl) trimethoxysilane进行化和化来激活聚烯表面.
- 通过 thiol 组的结合,粘附于氨酸覆盖的铜纳米颗粒.
- 使用谷物化交叉链接对西兰酶进行共价固定.
- 对酶活性,稳定性和在果汁澄清中应用的评估.
主要成果:
- 达到了 25.79% ± 0.82% 的静止收益率,用于 xylanase.
- 固定化西兰酶保留了大约77%的初始催化活性.
- 与可溶性西兰酶相比,已证明固定酶的热和pH稳定性得到了增强.
- 汁清晰度提高了29.9%,葡萄汁清晰度提高了26.9%.
结论:
- 开发的方法提供了一种高效的途径,用于使用功能化纳米粒子在聚乙烯上进行酶固定.
- 静止的西兰酶表现出优越的稳定性和水果汁澄清的显著潜力.
- 这种方法为食品工业中酶应用提供了一个有前途的策略.
相关概念视频
Fruit Development, Structure, and Function
25.2K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.2K
Pancreatic Juice and Secretion
3.0K
Pancreatic juice is a clear fluid produced by the pancreas, containing water, salts, sodium bicarbonate, and enzymes vital for digestion in the small intestine. It helps break down large molecules, facilitating nutrient absorption.
When acidic chyme from the stomach enters the duodenum, it triggers the release of secretin, a hormone that prompts pancreatic juice secretion. After a fatty meal, cholecystokinin, another hormone, stimulates gallbladder contraction and enhances enzyme-rich...
When acidic chyme from the stomach enters the duodenum, it triggers the release of secretin, a hormone that prompts pancreatic juice secretion. After a fatty meal, cholecystokinin, another hormone, stimulates gallbladder contraction and enhances enzyme-rich...
3.0K
Surface Tension and Surface Energy
3.2K
When a paint brush is immersed in water, the bristles wave freely inside the water. When it is taken out, the bristles stick together. The reason behind this effect is surface tension.
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...
3.2K
Wood Surfacing
353
Wood surfacing is a critical finishing process designed to smoothen the wood surface, enhance its dimensional accuracy, and make handling safer. This process compensates for potential shrinkage during the seasoning phase by marginally increasing the wood dimensions before surfacing. It also helps correct some distortions that may occur as the wood dries.
The equipment used in the surfacing process is a plane equipped with rotating blades. This tool efficiently smoothens the wood surface and can...
The equipment used in the surfacing process is a plane equipped with rotating blades. This tool efficiently smoothens the wood surface and can...
353
Cell-surface Signaling
54.3K
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
54.3K
Area of a Surface of Revolution
53
Surfaces of revolution are formed when a two-dimensional curve is rotated around an axis, producing a three-dimensional shape. This concept is used in engineering tasks like determining the surface area of a rocket nozzle, where precise calculations are critical for applying uniform heat-resistant coatings. When a curve is revolved about the x-axis, it sweeps out a continuous surface whose area must be calculated accurately to estimate material requirements.Approximating with Conical BandsTo...
53

