从零售肉中分离出来的肠道微生物的益生菌潜力的体外评估
Sangram Biswas1, Lutfor Rahman2, Md Taofiqur Rahman1
1Department of Biotechnology, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh.
PloS one
|January 28, 2026
概括
研究人员从肉中分离了四种乳酸细菌 (LAB) 菌株,确定了动物料中有希望的益生菌候选人. 这些有益的细菌增强了肠道健康和控制病原体,为抗生素提供了替代品.
科学领域:
- 动物科学动物科学
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
背景情况:
- 益生菌是有益的微生物,增强宿主肠道健康,并被探索为动物生产中抗生素的替代品.
- 乳酸细菌 (LAB) 是益生菌的一个关键组,在动物料中具有潜在的应用.
- 肉的胃肠道是分离新型益生菌菌株的丰富来源.
研究的目的:
- 从肉中分离,识别和表征具有益生菌性质的乳酸细菌 (LAB) 菌株.
- 评估这些LAB菌株作为改善动物健康和生产的料添加剂的潜力.
- 评估关键的益生菌特征,包括在模拟的胃条件下生存,胆盐耐受性和抗菌活性.
主要方法:
- 从肉GIT样本中分离微生物菌株,使用MRS和 agar.
- 通过格拉姆染色,催化酶测试和血液溶解活性测试对分离物的表征.
- 益生菌性质评估,包括对NaCl的耐受性,模拟胃汁,胆盐,抗生素易感性,细胞粘附性和16SrRNA基因测序用于鉴定.
主要成果:
- 隔离了40种微生物菌株,其中四种LAB菌株被确定为有前途的益生菌:Enterococcus faecium MCI7,Pediococcus pentosaceus MCI10,Pediococcus pentosaceus MCC6和Pediococcus pentosaceus MCC12.
- 选择的菌株表现出非溶血活性,在模拟胃汁中生存 (pH3) 和胆盐耐受性 (21.91-32.77%).
- 这些菌株对病原体 (10-16.5毫米抑制区),良好的细胞粘附性和对大多数测试的抗生素敏感性表现出显著的抗菌活性.
结论:
- 四种LAB菌株 (E.faecium MCI7,P.pentosaceus MCI10,P.pentosaceus MCC6,P.pentosaceus MCC12) 具有优异的益生菌性质.这些菌株的生长过程中,它们的生长过程中,它们的生长过程中,它们的生长过程中,它们的生长过程中,它们的生长过程中,它们的生长过程中.
- 这些已识别的LAB菌株是家禽料添加剂的有希望的候选者.
- 这些益生菌的使用可以帮助建立肠道菌群,提高肉质和生长,并控制肉的病原体.
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