乌拉功能化碳纳米管作为人工DNA链及其活体中毒性
Shwu-Chen Tsay1,2, Deepa R Landge1,2, Wen-Chieh Huang1,2
1Department of Chemistry, National Tsing Hua University, Hsinchu 300044, Taiwan.
ACS nano
|January 28, 2026
概括
功能化单壁碳纳米管 (f-SWCNTs) 显示了选择性DNA结合和伪结构的形成. 这些新型f-SWCNTs表现出高生物相容性和低毒性,显示出生物应用的前景.
科学领域:
- 纳米技术纳米技术
- 生物材料科学 生物材料科学
- 分子生物学分子生物学
背景情况:
- 单壁碳纳米管 (SWCNTs) 是具有生物应用潜力的多功能纳米材料.
- 用特定的连接体对SWCNT的功能化可以赋予独特的结合性质.
- 开发具有选择性DNA相互作用和生物相容性的纳米材料对于先进的应用至关重要.
研究的目的:
- 合成和表征具有DNA结合部分的功能化SWCNTs (f-SWCNTs).
- 研究f-SWCNTs与单链DNA (ssDNA) 和双链DNA (dsDNA) 的结合偏好.
- 评估开发的f-SWCNTs的生物相容性和生态毒性.
主要方法:
- 将SWCNT与具有DNA结合部分 (imidazolidin-2-one,hydantoin,urazole) 的功能化基基基基基基基基基基基接.
- 通过与ss-和dsDNA交织,形成伪双重和伪三重的DNA结构.
- 在不同pH值下使用ss-oligomers (dA25,dT25,dC25) 和duplex oligomers (dA25•dT25,dG25•dC25) 的杂交试验.
- 在C. elegans*上使用触摸反应和击试验进行生态毒性评估.
主要成果:
- 与 urazoles (1c) 功能化的 f-SWCNTs 显示强烈的结合ss-dA25和排他性的结合ss-dT25.
- ss-dC25 选择性地与 imidazolidin-2-one (1a) 和hydantoin (1b) 与f-SWCNTs结合.
- d(A•T) 对与1c有很强的结合,而 d(G•C) 对与1a有很强的结合.
- f-SWCNT 1c在酸性条件下表现出最强的结合 (pH 5.4).
- 缩短的f-SWCNT 1c对*C. elegans*没有显著的毒性,高达250微克/毫升,比SWCNT-COOH毒性低约2500倍.
结论:
- 功能化的SWCNT表现出强大的结合能力和核基选择性,特别是f-SWCNT 1c与 urazoles.
- 开发的f-SWCNTs可以形成稳定的伪双重和伪三重的DNA结构.
- 这些f-SWCNT具有很高的生物相容性,并且比未经修改的SWCNT毒性明显低,这表明具有广泛生物应用的潜力.
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