洞察红海伊里多病毒的病变发生:使用膜酵母双杂交和分子动态揭示宿主-病原体相互作用
Weichao Chen1, Jialong Yu1, Meijia He1
1State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
Bioorganic chemistry
|January 28, 2026
概括
红海伊里多病毒主要体蛋白与宿主HSC70相互作用,通过酵母查和AI结构预测确定. 这种相互作用对病毒病原和水产养殖中的潜在治疗点至关重要.
科学领域:
- 水生病毒学 水生病毒学.
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 红海皮病毒 (RSIV) 是对海洋水产养殖的重大威胁.
- RSIV的主要囊蛋白 (MCP) 是免疫原的,但其致病机制尚不清楚.
研究的目的:
- 为了阐明RSIV及其宿主之间的蛋白质相互作用.
- 了解RSIV病原性的分子基础.
主要方法:
- 使用膜酵母双杂交系统对RSIV-MCP检测红皮膜蛋白酵母cDNA库的选.
- 使用AlphaFold3进行蛋白质结构预测,然后进行分子对接和动力学模拟.
- 蛋白质与蛋白质相互作用的计算分析,包括结合和结合自由能计算.
- 突变分析以确定参与复杂形成的关键残留物.
主要成果:
- 确定了RSIV-MCP的53个潜在宿主相互作用伙伴,包括像HSC70.0.这样的热冲击蛋白.
- 产生了MCP和HSC70的高置信度结构模型,显示了稳定的复杂形成.
- 广泛的键和 -318.45 kJ/mol的结合自由能量表明MCP-HSC70的相互作用很强.
- 646ILE和452ILE的残留物被确定为复合物形成的关键.
结论:
- RSIV-MCP通过结和范德瓦尔斯力强烈地与宿主HSC70相互作用.
- 这些发现提供了对RSIV病原学的见解.
- 鉴定的相互作用为开发水产养殖中针对RSIV的治疗策略提供了潜在的目标.
相关概念视频
Yeast Signaling
17.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.3K
Hybridization of Atomic Orbitals I
66.9K
The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
66.9K
Molecular Models
43.7K
Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
43.7K
Hybridization of Atomic Orbitals II
48.8K
sp3d and sp3d 2 Hybridization
48.8K
Hybrid Zones
21.8K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
21.8K
Effect of Sea Water on Concrete
1.0K
Concrete exposed to seawater can undergo degradation like the dissolution of ettringite and gypsum, increasing the material's porosity and decreasing its strength. In contrast, the crystallization of salts within the concrete's pores can cause expansion, particularly above the waterline where evaporation occurs. Nonetheless, this expansion only happens when seawater, enabled by the concrete's permeability, manages to infiltrate the structure.
Concrete in areas between tide marks,...
Concrete in areas between tide marks,...
1.0K


