含有ZnO/Cu和生物活性玻璃纳米粒子的生物活性和抗菌通用粘合剂
Carolina Pardo-Díaz1, Gabriel Cochinski2, Pedro Moreira2
1Faculty of Dentistry, Dental School, University of Chile, 8380544, Santiago, Chile; Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, 84030-900, Ponta Grossa, Brazil; Department of Operative Dentistry, School of Dentistry, University of Valparaíso, 2360004, Valparaíso, Chile.
Journal of dentistry
|January 28, 2026
概括
生物活性玻璃纳米颗粒增强了通用粘合剂,改善了抗菌和矿化特性,而不损害细胞活力或键强度. 这为牙科修复材料提供了一种有前途的方法.
科学领域:
- 牙科材料科学 牙科材料科学
- 在牙科的纳米技术.
- 生物材料工程 生物材料工程
背景情况:
- 在现代牙科中,通用粘合剂对于将修复材料粘合到牙结构至关重要.
- 纳米颗粒的结合提供了增强粘合性质的潜力,如抗菌活性和矿化.
- 氧化 (ZnO),铜 (Cu) 和生物活性玻璃 (BG) 纳米颗粒正在研究它们的抗菌和生物活性潜力.
研究的目的:
- 评估ZnO,Cu和BG纳米粒子对通用粘合性质的影响.
- 评估了抗菌活性,细胞相容性,矿化,转化程度 (DC) 和树脂-丁丁微拉伸键强度 (μTBS).
- 研究了这些纳米粒子对矩阵金属蛋白酶 (MMP) 活性的影响.
主要方法:
- 测试了五种粘合剂配方,包括对照剂和ZnO/Cu和BG纳米粒子的各种组合.
- 量化了针对Streptococcus mutans的抗菌活性.
- 使用人类牙干细胞 (MTS试验) 评估了细胞兼容性.
- 在模拟体液浸泡后,通过FE-SEM和EDX评估矿化潜力.
- 用拉曼光谱测量DC,并根据自蚀刻 (SE) 和蚀刻和冲洗 (ER) 策略测试μTBS.
- 评估MMP活性是使用现场生殖图.
主要成果:
- BG纳米颗粒显著改善了抗菌作用和矿化,观察到超过80%的细菌抑制和显著的酸沉积.
- 所有测试的粘合剂都显示出良好的细胞兼容性 (>80%的细胞活力) 和适当的转化程度 (>60%).
- 在ER策略下,在含有BG的粘合剂中观察到微小的μTBS下降.
- 一般来说,MMP活性很高,在ER战略下,仅在ZnO/Cu组观察到轻微的减少.
结论:
- 将BG纳米粒子纳入ZnO/Cu修饰的通用粘合剂可以增强抗菌和矿化能力.
- 这些修改不会对细胞相容性或聚合物产生负面影响.
- 债券强度没有受到严重影响,尽管债券结合策略存在差异.
- MMP活性受到粘合策略的影响,而没有纳米粒子的一致抑制效应.
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