揭示小多脂纳米颗粒 (SMLPs) 的形态,由In-Vial同质化制成
Sevda Akcesme1, Stefanie Schmager1, Yassir Al-Tikriti2
1Institute of Pharmaceutical Sciences, University of Freiburg, 79104 Freiburg im Breisgau, Germany.
概括
小型多层脂质纳米颗粒 (SMLPs) 用双离心法制备. 研究了它们的形态学和水友性化合物捕获能力,揭示了对脂质相互作用和结构形成的见解.
科学领域:
- 脂质纳米颗粒制剂的配方
- 材料科学 材料科学 材料科学
- 药物输送系统 药物输送系统
背景情况:
- 热敏的小多层脂质纳米颗粒 (tSMLPs) 对温度触发的药物输送具有前景.
- tSMLP表现出独特的形态,具有紧密的双层和减少的膜间距.
- 之前的研究集中在tSMLP热敏度上;这项研究探讨了它们的形态.
研究的目的:
- 通过双离心法制备的小多层脂质纳米颗粒 (SMLPs) 的形态研究.
- 探索脂质组成 (DPPC/DPPG2) 对SMLP形成和特征的影响.
- 了解SMLP中含水性化合物被困的机制.
主要方法:
- 使用双离心法 (DC) 在高脂度 (60%) 中进行瓶内均质化,制备SMLP.
- 对DPPC/DPPG2摩尔比率 (x=0-100) 的系统选.
- 使用动态光散射 (DLS),时间分辨率光,小角度X射线散射 (SAXS),广角X射线散射 (WAXS) 和冷电子显微镜 (cryo-EM) 的表征.
主要成果:
- 200纳米以下的SMLP的形成,在各种DPPC/DPPG2比率中尺寸分布狭窄.
- 尽管有多层层结构,但SMLP已证明能够捕获水友化合物.
- 在DC过程中DPPC/DPPG2头组相互作用在水流入和囊泡脂凝 (VPG) 稀释中的假设作用.
结论:
- 双离心法对于制备可控制形态的SMLP是有效的.
- 脂质头组相互作用显著影响SMLP结构和水的捕获.
- 该研究提供了一个基于DPPG2内容的SMLP形态演变的假设.
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