Circ_0075691通过与EIF4A3相互作用来调节粒粉细胞中的脂质代谢,以促进PTGS2mRNA的稳定性
Pengyu Huang1, Jianshu Cai2, Gangxin Chen3
1Fujian Provincial Human Sperm Bank, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, 350001, China.
Biochimica et biophysica acta. Molecular basis of disease
|January 28, 2026
概括
循环RNAcirc_0075691通过损害粒粉细胞功能和脂质代谢,加剧了多囊卵巢综合征 (PCOS). 针对circ_0075691/EIF4A3/PTGS2通路可能为PCOS提供新的治疗策略.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 多囊卵巢综合征 (PCOS) 与循环RNAs (circRNAs) 有关,但它们在颗粒状细胞功能障碍中的特定作用尚未完全理解.
- 这项研究研究了circ_0075691在PCOS相关的代谢障碍中的功能.
研究的目的:
- 阐明circ_0075691在PCOS相关的粒状细胞功能障碍和代谢失调中的机制性作用.
- 为了确定涉及PCOS中circ_0075691的分子相互作用和途径.
主要方法:
- 使用凝电泳,RNase R试验,RT-PCR和FISH证实了circ_0075691的圆形结构和核定位.
- 通过细胞测定,评估circ_0075691过度表达对颗粒细胞功能,氧化应激和脂质代谢的影响.
- 验证了circ_0075691与EIF4A3的相互作用及其对PTGS2mRNA稳定性的影响,使用RIP和RNA拉向测定.
主要成果:
- 在PCOS粒状细胞和毛囊液中,Circ_0075691的表达显著上调.
- 过度表达circ_0075691会损害细胞活力,增殖和代谢功能,同时增加细胞亡和脂质积累.
- Circ_0075691稳定了EIF4A3,增强了PTGS2的mRNA稳定性,而沉默PTGS2则改善了PCOS相关的细胞异常.
结论:
- Circ_0075691通过破坏颗粒细胞功能和脂质代谢,在PCOS病变发生过程中发挥着关键作用.
- 已确定的circ_0075691/EIF4A3/PTGS2轴为PCOS提供了新的分子洞察力.
- 这一途径代表了PCOS治疗的潜在治疗标.
相关概念视频
02:22Regulated mRNA Transport
7.0K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.0K
02:22Regulated mRNA Transport
3.4K
3.4K
02:51mRNA Stability and Gene Expression
6.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
6.6K
02:51mRNA Stability and Gene Expression
3.5K
3.5K
02:41Chromatin Structure Regulates pre-mRNA Processing
8.2K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
8.2K
01:19Regulation of Metabolism
11.6K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
11.6K