Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Phosphorylation01:02

Phosphorylation

53.8K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
53.8K
Cycloaddition Reactions: MO Requirements for Thermal Activation01:16

Cycloaddition Reactions: MO Requirements for Thermal Activation

4.4K
Thermal cycloadditions are reactions where the source of activation energy needed to initiate the reaction is provided in the form of heat. A typical example of a thermally-allowed cycloaddition is the Diels–Alder reaction, which is a [4 + 2] cycloaddition. In contrast, a [2 + 2] cycloaddition is thermally forbidden.
4.4K
Cycloaddition Reactions: MO Requirements for Photochemical Activation01:12

Cycloaddition Reactions: MO Requirements for Photochemical Activation

2.7K
Some cycloaddition reactions are activated by heat, while others are initiated by light. For example, a [2 + 2] cycloaddition between two ethylene molecules occurs only in the presence of light. It is photochemically allowed but thermally forbidden.
2.7K
Energy-requiring Steps of Glycolysis01:20

Energy-requiring Steps of Glycolysis

171.5K
Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
171.5K
Requirements for Human Life01:26

Requirements for Human Life

13.5K
The Earth and its atmosphere have provided humans with air, water, and food, but these are not the only requirements for survival. Humans also require a specific range of temperature and pressure that the Earth and its atmosphere provides.
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...
13.5K
Proteins: Dietary Sources and Requirements01:28

Proteins: Dietary Sources and Requirements

1.8K
Consuming animal-based products offers high-quality proteins that contain optimal levels and combinations of essential amino acids, crucial for tissue repair and growth. Foods like eggs, milk, fish, and most meats are a source of complete proteins. Legumes and cereals are abundant in proteins; however, they typically lack a full range of essential amino acids. As a result, they are considered incomplete protein sources. Some plant sources like soybeans, quinoa, and amaranth do contain complete...
1.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Molecular anatomy of PLK1 master docking motifs.

Nature communications·2026
Same author

Molecular basis of cooperative assembly of the Ndc80-Ska kinetochore complex on microtubules.

bioRxiv : the preprint server for biology·2026
Same author

Molecular tweezer-peptide conjugates disrupt the protein-protein interaction between survivin and histone H3 essential in mitosis.

Beilstein journal of organic chemistry·2026
Same author

Ancient co-option of LTR retrotransposons as yeast centromeres.

Nature·2026
Same author

M18BP1 valency and a distributed interaction footprint determine epigenetic centromere specification in humans.

The EMBO journal·2026
Same author

CUL4A-DDB1-DCAF10 is an N-recognin for N-terminally acetylated Src kinases.

Nature communications·2026

相关实验视频

Updated: Jan 30, 2026

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

12.5K

通过T环酸化激活PLK1的分子要求.

Arianna Esposito-Verza1,2, Duccio Conti3, Paulo D Rodrigues Pedroso3,4

  • 1Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, Dortmund, 44227, Germany. arianna.espositoverza@mpi-dortmund.mpg.de.

The EMBO journal
|January 28, 2026
PubMed
概括

主性线粒激酶PLK1需要酸化才能激活. 这项研究表明,Aurora A的合作伙伴玻拉对PLK1 Thr210酸化是唯一必要的,揭示了关键的特异性机制.

关键词:
在这里,我们可以看到AuroraAuroraAurora.细胞循环 细胞循环基因酶激酶的使用方法波罗类类型的激酶 (Polo-like Kinase) 是一种动态图片 动态图片

更多相关视频

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
08:55

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

16.1K
Modeling an Enzyme Active Site using Molecular Visualization Freeware
14:37

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Published on: December 25, 2021

11.5K

相关实验视频

Last Updated: Jan 30, 2026

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

12.5K
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
08:55

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

16.1K
Modeling an Enzyme Active Site using Molecular Visualization Freeware
14:37

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Published on: December 25, 2021

11.5K

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 波罗样酶1 (PLK1) 是线索分裂的一个关键调节剂.
  • PLK1的激活取决于Thr210的酸化,Thr210通常是Aurora激酶的目标.
  • 之前的研究提出了有关负责Thr210酸化的特定Aurora激酶的相互矛盾的证据,突出了Aurora A合作伙伴Bora的作用.

研究的目的:

  • 阐明PLK1 Thr210酸化中的要求的机械基础.
  • 为了确定负责PLK1激活的特定光激酶复合体.
  • 了解玻拉和PLK1之间促进酸化的相互作用.

主要方法:

  • 在试验室中使用纯化的蛋白质进行激酶测试 (Aurora A,Aurora B,Bora,PLK1).
  • 针对PLK1的位点定向突变发生,以探测相互作用的要求.
  • 结构建模用于预测蛋白质相互作用.
  • 生物化学测试以评估酶活性和基质酸化.

主要成果:

  • 在体外,Aurora A复合与玻拉特异性化PLK1 Thr210的Bora.
  • 隔离的Aurora A,其他Aurora A复合体,以及Aurora B:INCENP都未能酸化PLK1 Thr210.
  • 通过结构建模和突变分析证实,和PLK1之间的短暂相互作用对Thr210酸化至关重要.
  • 在PLK1中将Lys208转化为氨酸取消了玻拉的依赖性,使PLK1成为多个光激酶的基质.

结论:

  • 罗拉A:博拉复合体是通过Thr210酸化激活PLK1的主要酶.
  • 玻拉和PLK1之间的特定相互作用决定了PLK1激活的激酶特异性.
  • 了解这种机制为调节线粒激酶和细胞循环控制提供了新的见解.