3D表观基因组架构编排米花期的cis和trans调节
Yue Li1, Zhanyi Zhang2, Leilei Qiu3
1National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.
Genome biology
|January 29, 2026
概括
研究人员绘制了大米的3D基因组图,揭示了素三甲基化 (H3K27me3) 如何调节开花时间基因. 一个关键的发现是由相隔抑制剂控制的开花基因的空间集群.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 基因组H3氨酸27三甲基化 (H3K27me3) 建立了对植物发育至关重要的抑制色素状态.
- 与H3K27me3相关的三维 (3D) 基因组架构及其在米发育中的作用尚不清楚.
研究的目的:
- 为了研究大米中H3K27me3的3D表观基因组拓.
- 了解H3K27me3相关染色体结构在水开花时间中的调节作用.
主要方法:
- 通过对结尾标签测序 (ChIA-PET) 进行长读色素相互作用分析,用于绘制H3K27me3相关基因组拓.
- 进行了OsEMF2b的T-DNA插入性突变发生,以评估其在染色质相互作用中的作用.
- 液-液相分离和结合试验被用来研究开花调节剂.
主要成果:
- 在大米基因组中发现了数百个压制性染色质空间集群.
- OsEMF2b 干扰改变了 H3K27me3 染色体相互作用网络.
- 关键的开花时间基因 (Ehd1,Hd3a/RFT1) 形成了一个染色体间抑制集群.
- Ghd7,Ghd8和Hd1被确定为跨作用的开花调节剂,通过相位分离与此集群结合.
结论:
- 在米中阐明了H3K27me3相关位点的多维拓框架.
- 一个由相分离抑制器复合体 (Ghd7,Ghd8,Hd1) 调节的空间开花基因集群被确定.
- 这些发现增强了对3D基因组在米中协调开花时间基因 (florigens) 的作用的理解.
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