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尼提丁化物介导的抗结直肠癌活动的机制性调查:通过综合分子动力学,空间转录组和单细胞方法来准与中心分子相关的蛋白质E
Bin Li1, Zhi-Su Liu1, Ke-Jun Wu1
1Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
IET systems biology
|January 29, 2026
概括
尼提丁化物 (NC) 准了与中间体相关的蛋白质E (CENPE) 来对抗结直肠癌 (CRC). 这项研究揭示了NCNC.
科学领域:
- 瘤学和药理学方面的研究.
- 分子生物学分子生物学
- 传统中国医学 (TCM) 是一种
背景情况:
- 结肠直肠癌 (CRC) 是全球癌症相关死亡的主要原因.
- 常规化疗对于CRC往往导致严重的副作用和耐药性.
- 传统中医 (TCM) 由于其多组分和多目标协同效应,为新型抗癌疗法提供了一个有前途的途径.
研究的目的:
- 研究尼提丁化物 (NC) 的抗CRC作用背后的分子机制,尼提丁化物 (NC) 是来自Zanthoxylum nitidum的生物活性化合物.
- 探索细胞循环调节剂的中心相关蛋白E (CENPE) 作为CRC.中潜在的治疗点的作用.
- 用TCM衍生化合物为CRC开发精密疗法建立科学基础.
主要方法:
- 综合单细胞RNA测序 (scRNA-seq),空间转录组学,大规模mRNA队列分析和免疫组织化学 (IHC) 以评估CRC中的CENPE表达.
- 利用实时定量聚合酶链反应 (RT-qPCR) 和IHC来评估NC对CENPE表达的影响.
- 采用分子动力学模拟 (MDS) 来分析NC和CENPE之间的结合相互作用.
主要成果:
- 发现CENPE在CRC组织中显著过度表达,恶性区域表达得分高.
- 克里斯普尔查表明,CENPE淘汰显著降低了CRC细胞的增殖.
- MDS预测NC和CENPE之间的稳定结合模式,主要由范德瓦尔斯相互作用驱动.
- 在CRC细胞和异种移植模型中,NC治疗导致CENPE mRNA和蛋白质水平的显著下调.
结论:
- 尼提丁化物 (NC) 通过向中粒素相关蛋白E (CENPE) 来发挥抗结肠直肠癌的活性.
- 这项研究为精确的CRC疗法提供了机制基础,利用传统中医药的活性成分.
- 这些发现表明了结直肠癌治疗的新疗法策略.
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