一种临床生物标志物试验,用于定量人类血清中CXCL9的数量
Lucia Franco-Dilone1, Oitak Allen Wong1, Omar F Laterza1
1Translational Molecular Biomarkers, Merck & Co., Inc, Rahway, NJ, USA.
BioTechniques
|January 29, 2026
概括
一种新的测定方法可靠地测量人血清中的CXCL9,使其能够在临床试验中作为生物标志物用于预测治疗反应.
科学领域:
- 生物化学 生物化学
- 临床化学 临床化学
- 免疫学 免疫学 免疫学
背景情况:
- 循环中的CXCL9是临床研究的潜在生物标志物.
- 试验验证对于可靠的生物标志物评估至关重要.
研究的目的:
- 验证人类血清CXCL9.9的电化学发光试验.
- 确定测试性能特征,包括灵敏度,精度和准确性.
- 评估血清中CXCL9的生物变异性和稳定性.
主要方法:
- 验证了人类血清的电化学发光免疫试验.
- 优化测定条件以减轻矩阵效应.
- 确定量化的下限 (LLOQ),精度和准确性.
- 评估生物变异性和分析物的稳定性.
主要成果:
- 经过验证的试验表明超灵敏度的LLOQ为1.64 pg/mL.
- 测试在人类血清中表现出最佳的精度和准确性.
- 生物变异性和CXCL9稳定性的特征.
结论:
- 一种经过验证的,超敏感的CXCL9测定方法适用于探索性生物标志物的使用.
- 这种测试可以评估CXCL9作为临床研究中的药理动力学或预测标记.
- 该试验支持CXCL9水平与临床反应的相关性.
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