在扩展显微镜中使用3D-Aligner纠正图像扭曲
Wan-Yi Hsiao1, Dhaval Ghone1,2, Aussie Suzuki1,2,3
1McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin-Madison, Madison, WI, USA.
Bio-protocol
|January 29, 2026
概括
扩展显微镜 (ExM) 中的样本漂移阻碍了3D成像. 我们开发了3D-Aligner软件以计算纠正这种漂移,确保精确的纳米尺度可视化和ExM和其他光显微镜数据集的定量分析.
科学领域:
- 显微镜和成像技术技术.
- 生物技术和生物成像技术
- 计算生物学 计算生物学
背景情况:
- 扩展显微镜 (ExM) 通过物理扩大样本提供超高分辨率成像,克服纳米生物可视化的衍射极限.
- 尽管取得了进展,但ExM成像准确性受到采集过程中样本漂移的影响,扭曲了3D重建和定量分析.
- 现有的方法很难准确地纠正各种ExM数据集中的漂移,其扩展因子和标签各不相同.
研究的目的:
- 开发和验证用于纠正ExM数据集样本漂移的计算工具.
- 提高超分辨率显微镜3D重建和定量分析的可靠性.
- 为ExM和传统光显微镜提供一种易于使用的解决方案.
主要方法:
- 开发了3D-Aligner,这是一款利用背景特征检测和跨图像堆匹配来确定漂移轨迹的软件.
- 实现了纳米尺度对齐算法以计算纠正样本运动.
- 验证了软件在不同扩张因子和光标签的ExM数据集上的性能.
主要成果:
- 3D-Aligner准确地纠正ExM数据集中的样本漂移,恢复结构忠实度.
- 该软件在各种扩展因子和标签条件中展示了强大的性能.
- 3D-Aligner的性能优于传统的注册工具,适用于非ExM光显微镜数据.
结论:
- 3D-Aligner提供了一个精确且用户友好的解决方案,用于减轻ExM成像中的样本漂移.
- 这种计算方法使得可靠的3D重建和纳米生物结构的定量评估.
- 该软件增强了ExM和其他光显微镜技术在先进生物研究中的实用性.
关键词:
3D重建重建的3D重建3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D-Aligner 3D在3D-Speckler中使用3D-Speckler.计算机成像成像技术漂移纠正 漂移纠正 漂移纠正扩展显微镜 (ExM) 的使用图像分析 图像分析图像扭曲的纠正 图像扭曲的纠正图像的注册 图像的注册
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