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使用RPA-PfAgo系统加上光和侧向流量测量棒的快速和特定检测Streptococcus suis2血清型

Kexin Wang^1,2, Xujie Zhao^1,2, Jingjing Li^1,2

¹College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China.

Frontiers in veterinary science

|January 29, 2026

概括

使用重组酶聚合酶放大 (RPA) 和Pyrococcus furiosus Argonaute (PfAgo) 的新双检测平台可以快速准确地识别Streptococcus suis血清型2. 这些测试适用于实验室和农场测试.

科学领域:

分子生物学分子生物学
生物技术是生物技术.
兽医诊断兽医诊断兽医诊断兽医诊断

背景情况:

葡萄球菌 suis 血清型 2 (SS2) 是一个重要的猪病原体.
准确和快速的诊断方法对于控制SS2疫情至关重要.
现有的诊断方法可能缺乏速度或现场适用性.

研究的目的:

开发和验证将复合酶聚合酶放大 (RPA) 与Pyrococcus furiosus Argonaute (PfAgo) 集成的双检测平台.
为了实现快速和特定的Streptococcus suis血清型2 (SS2) 的识别.
建立适合实验室和护理场所环境的测试方法.

主要方法:

保存的cps2J基因是SS2检测的目标.
重组酶聚合酶放大 (RPA) 和PfAgo反应条件得到了优化.
用各种标准和临床样本评估试验特异性和灵敏性,与国家标准PCR试验进行比较.

主要成果:

开发了两个格式:实时光 (RPA-PfAgo-RTF) 和侧流量计 (RPA-PfAgo-LFD).
RPA-PfAgo-RTF测定检测到10^0副本/μL,而RPA-PfAgo-LFD检测到10^2副本/μL.
两种测定都显示出高特异性,在41个临床样本上100%与PCR一致,总检测时间小于1小时.

结论:

开发的RPA-PfAgo双检测平台提供快速,灵敏和准确的SS2识别.
这些测试非常适合用于实验室诊断.
这些平台还有效用于农场的临床检测,有助于疾病管理.

关键词:

这是PfAgo.链球菌 suis 血清型 2 类型光检测的检测方法侧向流量测量棒横向流量测量棒在医疗保健中心进行检测.快速的诊断快速的诊断快速的诊断复合酶聚合酶放大复合酶.

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