昆蛋白质水解剂:抗氧化特性和细胞保护,防止D-银糖诱导的氧化应激
Cheng Yang1, Chao Yang1, Yuanyuan Zhou1
1Agricultural Product Storage and Processing Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Frontiers in nutrition
|January 29, 2026
概括
酶可以有效地产生抗氧化类蛋白质化物 (QPH),具有高必需氨基酸含量和强大的激素清除活性. 这些QPH显示出作为功能性食品和营养食品的天然抗氧化剂的前景.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 营养保健品 营养保健品
背景情况:
- 昆蛋白是具有健康益处的生物活性的宝贵来源.
- 酶性水解是生产具有增强功能的蛋白质水解盐的关键方法.
- 了解蛋白酶特异性对于优化华蛋白质水解剂 (QPHs) 中抗氧化物质的生成至关重要.
研究的目的:
- 为了比较四种蛋白质酶 (酶,素,素,中性蛋白质酶) 在产生抗氧化剂QPHs中的有效性.
- 为了确定最佳的酶条件,最大限度地提高抗氧化剂的潜力和生物活性产量从子蛋白.
- 评估由不同蛋白酶产生的QPH的功能性质,包括激素清除和细胞保护作用.
主要方法:
- 在不同的条件下,使用酸盐,素,素和中性蛋白酶来化奎诺亚蛋白.
- 分析了水解程度,分子量分布,表面疏水性和必需氨基酸含量.
- 使用DPPH,ABTS和FRAP试验,以及在HepG2细胞中的细胞保护试验来评估抗氧化活性.
主要成果:
- 酸产生了最高的水解度 (36.15%) 和最大的低分子量 (<2kDa) 分数.
- 酶衍生的QPH在HepG2细胞中表现出优异的DPPH激素清除 (4.30 mg TE/g),降低功率 (0.61) 和细胞保护 (83.43%).
- 素水解有选择地增强了ABTS的激素清除 (7.22 mg TE/g) 和FRAP值 (13.26 μmol TE/100 g DW).
结论:
- 酸酶是最有效的蛋白酶,用于产生具有高必需氨基酸含量和广泛生物活性的抗氧化剂QPH.
- 酶性衍生QPHs,特别是酸盐,是功能性食品和营养食品的有希望的天然成分.
- 这项研究提供了QPH抗氧化特性的机械洞察力,支持其在促进健康的产品中的应用.
相关概念视频
03:14Oxidation Numbers
42.5K
In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.5K
02:58Properties of Transition Metals
29.8K
Transition metals are defined as those elements that have partially filled d orbitals. As shown in Figure 1, the d-block elements in groups 3–12 are transition elements. The f-block elements, also called inner transition metals (the lanthanides and actinides), also meet this criterion because the d orbital is partially occupied before the f orbitals.
29.8K
01:15Pyruvate Oxidation
168.8K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
168.8K
03:11Oxidation-Reduction Reactions
75.6K
Oxidation–Reduction Reactions
75.6K
02:45Responses to Heat and Cold Stress
14.8K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
14.8K
02:57Physical and Chemical Properties of Matter
166.2K
The characteristics that enable us to distinguish one substance from another are called properties.
166.2K