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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Hydrogen Bonds

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Hydrogen bonds are weak attractions between atoms that have formed other chemical bonds. One of these atoms is electronegative, like oxygen, and has a partial negative charge. The other is a hydrogen atom that has bonded with another electronegative atom and has a partial positive charge.
Hydrogen Bonds Control the World!
Because hydrogen has very weak electronegativity when it binds with a strongly electronegative atom, such as oxygen or nitrogen, electrons in the bond are unequally shared....
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Hydrogen Bonds

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A hydrogen bond is formed when a weakly positive hydrogen atom already bonded to one electronegative atom (for example, the oxygen in the water molecule) is attracted to another electronegative atom from another polar molecule, such as water (H2O), hydrogen fluoride (HF), or ammonia (NH3). The huge electronegativity difference between the H atom (2.1) and the atom to which it is bonded (4.0 for an F atom, 3.5 for an O atom, or 3.0 for an N atom), combined with the very small size of an H atom...
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According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
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Machines are complex structures consisting of movable, pin-connected multi-force members that work together to transmit forces. One example of a machine is the cutting plier, which is used to cut wires by applying forces to its handles. When equal and opposite forces are exerted on the handles of the cutting plier, they cause the cutting edges to come together and apply equal and opposite reaction forces on the wire, which are greater than the applied forces.
A free-body diagram of the...
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When a wave travels from one medium to another, it gets reflected at the boundary of the second medium. A common example of this is when a person yells at a distance from a cliff and hears the echo of their voice. The sound waves (longitudinal waves) traveling in the air are reflected from the bounding cliff. Similarly, flipping one end of a string whose other end is tied to a wall causes a pulse (transverse wave) to travel through the string, which gets reflected upon reaching the wall. In...
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使用反射显微镜和机器学习检测透的新方法.

Aleksei Makogon1, Frédéric Kanoufi1, Varvara Helbert2

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我们开发了一种使用反射显微镜和机器学习检测钢中的的新方法. 该技术精确地识别金属膜中受影响的区域,这对于材料科学应用至关重要.

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科学领域:

  • 材料科学 材料科学 材料科学
  • 分析化学 分析化学
  • 表面科学是一门学科.

背景情况:

  • 在工业应用中,局部检测钢膜中的透性至关重要.
  • 目前分析透率的方法通常范围或可访问性有限.
  • 了解与钢的相互作用对于防止材料降解至关重要.

研究的目的:

  • 开发和验证一种用于局部检测和量化钢膜中透的新型框架.
  • 提高光学方法的灵敏度,以检测由引起的微妙变化.
  • 为现有的分析技术提供一种可访问和有效的替代方案.

主要方法:

  • 使用反射显微镜 (RM) 来捕捉钢表面的变化.
  • 应用机器学习 (ML) 驱动的图像分析,包括UMAP和K-means集群,用于对比度增强和特征检测.
  • 采用时间序列分析来放大与存在有关的微妙光学信号.
  • 使用扫描凯尔文探测器 (SKP) 测量的验证结果.

主要成果:

  • 成功检测出由于透而引起的改变Fe (II) /Fe (III) 比率引起的反射光强度的微妙变化.
  • 该RM-ML框架有效地增强了对比度,使近光学极限信号可检测.
  • 在钢膜中确定了高精度的特定受影响的区域.
  • 通过独立的SKP测量证实了RM-ML的发现.

结论:

  • 建立了一个新的反射显微镜机器学习 (RM-ML) 框架,用于在金属膜中局部检测.
  • 展示了这种可访问技术的潜力,作为分析透的强大工具.
  • 这种方法为材料中的定量,局部分析提供了一个有希望的替代方案.