三种替代性促进体控制中的PGC-1α表达,并直接被PPARγ抑制
Jiaxin Huang1, Fang Mu1, Ming Lou1
1Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
的PGC-1α基因转录由三个替代促进体调节. 过氧体增殖器激活受体-γ (PPARγ) 负面控制这些促进体和PGC-1α异型体的表达.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 动物科学动物科学
背景情况:
- 过氧体增殖器激活的受体-γ协同激活剂-1α (PGC-1α) 是哺乳动物的一个关键的转录协同激活剂.
- 中的PGC-1α的基因结构和调节机制在很大程度上是未知的.
研究的目的:
- 为了阐明PGC-1α基因的转录调节.
- 调查氧酶增殖器激活受体-γ (PPARγ) 在调节的PGC-1α表达中的作用.
主要方法:
- 使用5'快速放大cDNA末端 (5'RACE) 识别了PGC-1α基因的三个明显的第一个前基因.
- 通过使用双露西法酶报告员测定来评估促进者活动,并通过生物信息学识别了PPAR响应元素 (PPRE).
- 通过过度表达和敲击实验研究了PPARγ的调节作用,通过染色体免疫沉 (ChIP) 试验证实了结合.
主要成果:
- 鉴定了PGC-1α的三个替代促进体 (P1,P2,P3),每一个都显示出显著的促进体活性.
- 发现PPARγ可以负面调节所有三种促进体以及PGC-1α异型体 (cPGC-1α1,cPGC-1α2,cPGC-1α3) 的表达.
- 活体中证实了PPARγ与促进子区域的结合,并且观察到cPGC-1α异型的独特表达模式,与选择的 brojler线中的PPARγ相反相关.
结论:
- 的PGC-1α转录是由三个替代性促进体控制的.
- 通过这些促进体,PPARγ作为PGC-1α转录的负调节者.
- 这些发现为的PGC-1α调节提供了洞察力,这与脂肪代谢和家禽科学有关.
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