双CRISPR/Cas驱动的无放大表面增强的拉曼散射生物传感器与智能手机相结合,可同时检测总和活目标细菌
Ruibao Jiao1, Qun Ni2, Ruirui Zhao2
1Department of Laboratory Medicine, Tongling People's Hospital, Tongling, Anhui, 244000, PR China; Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, Anhui, 230032, PR China.
Biosensors & bioelectronics
|January 29, 2026
概括
这项研究引入了一种新的双CRISPR/Cas驱动生物传感器,用于同时检测总和活细菌. cc-SERS生物传感器使用独特的拉曼信号区分活体和死体细菌,从而实现快速现场检测.
科学领域:
- 生物技术是生物技术.
- 生物感应是一种生物感应.
- 分子诊断学 分子诊断
背景情况:
- 同时检测总和活细菌数量至关重要,但在技术上具有挑战性.
- 现有的方法往往缺乏实时监控所需的特异性或速度.
- 区分活细菌和死细菌对于准确的感染评估和控制至关重要.
研究的目的:
- 开发一种新型的生物传感器,用于同时无放大检测总和活细菌.
- 利用CRISPR/Cas系统和表面增强的拉曼散射 (SERS) 进行增强的细菌检测.
- 创建一个快速的,可在现场部署的细菌定量诊断工具.
主要方法:
- 设计了一种双重的CRISPR/Cas12a和CRISPR/Cas13a系统,利用差异性的DNA和RNA在细菌死亡后的稳定性.
- 表面增强拉曼散射 (SERS) 用于信号生成和检测,用于DNA和RNA激活有明显的拉曼转移.
- 快速预处理方法和智能手机辅助的便携式拉曼光谱仪被整合为现场可用性.
主要成果:
- cc-SERS生物传感器使用特定的拉曼信号 (分别为1079厘米-1和593厘米-1) 成功区分了总 (基于DNA) 和活 (基于RNA) 的细菌.
- 在总细菌和活细菌中达到约10 CFU/mL的低检测极限.
- 在现场条件下在45分钟内检测出黄金葡萄球菌和Sakazakii黑菌的成功应用.
结论:
- 开发的cc-SERS生物传感器为同时检测总和活细菌提供了一个有前途的解决方案.
- 没有放大,双CRISPR/Cas方法提供高特异性和灵敏度.
- 这项技术在各种应用中具有快速的现场细菌监测的巨大潜力.
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