开发一种石墨烯氧化物-SELEX衍生的阿普坦和一个侧流生物传感器,用于现场视觉检测异-甲基
Mingxuan Fang1, Yinghui Wang1, Yifei Qin2
1College of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Food chemistry
|January 29, 2026
概括
研究人员开发了一种新型的aptamer生物传感器,用于检测有毒农药异芬-甲基 (IFP). 这种具有成本效益的测试带可以在蔬菜中快速准确地检测IFP,从而加强食品安全监测.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 伊索芬甲基 (IFP) 是一种高度有毒的有机农药,具有显著的食品安全风险.
- 现有的IFP检测方法可能耗时且复杂.
研究的目的:
- 为了开发高亲和度的aptamers特定于isofenphos-methyl.
- 为在食品样本中检测IFP创建一个快速,经济高效的基于aptamer的生物传感器.
主要方法:
- 石墨烯氧化物-SELEX被用来为IFP选择高亲和度的受体.
- 一个横流带生物传感器是使用aptamers和金纳米粒子进行工程的.
- 聚乙烯胺被用作信号放大测试线材料.
主要成果:
- 选择了一种78-nt的亚胺,其Kd值为21.70±2.53nM.
- 体生物传感器显示了广泛的检测范围 (20-300 nM) 和低检测极限 (12.61 nM).
- 对尖蔬菜的分析显示了高恢复率 (90.67%-104.63%) 和良好的精度 (RSD<5.29%).
结论:
- 成功开发了一种新的aptamer侧流带生物传感器,用于快速检测IFP.
- 生物传感器对于农业安全监测非常实用,可提供视觉半定量分析.
- 这种方法为在现实世界样本中检测IFP提供了具有成本效益和易于操作的解决方案.
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