通过超声波辅助的pH转移进行蛋黄颗粒的界面重组:度依赖于莱西丁的功能从乳液稳定剂到竞争性取代剂
Shuangshuang Li1, Yaqin Yang1, Yi Sun1
1National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Food chemistry
|January 29, 2026
概括
通过添加卵黄素 (EL) 和超声波辅助的pH转移,改善了蛋黄颗粒 (EYG) 乳化. 最佳EL度 (0.5%) 创造了稳定的乳液,增强了食品的功能.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生化学
- 材料科学 材料科学 材料科学
背景情况:
- 蛋黄颗粒 (EYG) 具有密集的结构,限制了它们的乳化特性.
- 改善EYG乳化对于开发新型食品成分和稳定乳液至关重要.
研究的目的:
- 为了研究卵子莱素 (EL) 和超声波辅助的pH转移对卵黄颗粒 (EYG) 功能的协同作用.
- 阐明EYG-EL相互作用背后的分子机制,以增强乳化.
主要方法:
- 超声波辅助的pH值转移与不同度的蛋叶素 (0-1%) 和蛋黄颗粒 (1%) 相结合.
- 使用光谱学 (FTIR,光) 分析复杂可溶性,泽塔潜力,粒子大小和结构变化.
- 分子对接模拟用于预测蛋白质-脂相互作用和界面层特性.
主要成果:
- 蛋素显著改善了EYG溶解度,增加了负Zeta潜力,并减少了颗粒大小 (P<0.05).
- 结构分析显示,由于EL,蛋白质中的β-sheet含量增加,随机线圈减少 (P <0.05).
- 最佳0.5%的EL度促进了蛋白质-脂协同作用,形成具有小滴粒大小 (9.55微米) 和低度 (3.64%) 的稳定乳液.
- 较高的EL度 (>0.5%) 导致竞争性吸附,削弱界面稳定性.
结论:
- 蛋莱西丁和超声波辅助的pH转移协同增强了EYG乳化功能的功能.
- 蛋白质-脂相互作用是形成稳定的界面层的关键,以最佳的EL度为最大的乳液稳定性.
- 这项研究为设计基于EYG和EL的功能乳化剂提供了机械洞察力,用于定制的应用.
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