针对乳腺癌放射光热治疗的HER-2向的金纳米的表征
Omid Talaee1, Mahboobeh Mehrabifard2, Bahman Alipour3
1Department of Nuclear Engineering, Faculty of Mechanical Engineering, University of Shiraz, Shiraz, Iran.
装有Trastuzumab的金纳米 (TZ-Au@SiO2 NSs) 在改善乳腺癌治疗方面表现有前途. 将这些纳米粒子与激光和X射线辐射相结合,显著降低了癌细胞活力,表明了改善治疗的潜力.
科学领域:
- 纳米技术纳米技术
- 生物医学工程 生物医学工程
- 在瘤学瘤学.
背景情况:
- 纳米粒子 (NP) 与放射和化疗相结合,可提供增强的乳腺癌 (BC) 治疗.
- 这项研究侧重于用于HER-2+和HER-2-BC细胞的Trastuzumab装载的Au@SiO2纳米 (TZ-Au@SiO2 NSs).
研究的目的:
- 研究TZ-Au@SiO2 NSs在乳腺癌治疗中的治疗潜力.
- 为了评估TZ-Au@SiO2 NSs与激光和X射线辐射的联合疗效.
主要方法:
- 使用酸盐还原和sol-gel方法合成的Au@SiO2NP,与Trastuzumab (TZ) 相结合.
- 通过FT-IR,DLS,TEM,SEM,UV-Vis,XRD进行表征;评估药物加载效率.
- 治疗后分析了细胞活力 (MTT测定) 和与亡相关的基因表达 (RT-qPCR).
主要成果:
- 在TZ-Au@SiO2 NSs (大约. 70nm) 显示出显著的温度升高能力.
- 使用TZ-Au@SiO2 NSs,激光和X射线的联合治疗显著降低了SKBr-3和MCF-7细胞的细胞活力.
- 基因表达分析显示TP53的上调调节,这表明亡诱导.
结论:
- TZ-Au@SiO2 NSs增强了X射线辐射和光热疗法的细胞毒性作用.
- 这些纳米显示出作为放射敏感剂和光敏感剂的潜力,用于改善乳腺癌治疗,特别是SKBr-3细胞.
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