快速液体活检评估通过从脏活检输送介质的基因分析:技术验证和概念验证试点研究
Ziyang Li1, Marij J P Welters2, Hailiang Mei3
1Department of Pathology, and Leiden Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.
概括
活检输送介质 (BTM) 可以加速病诊断. 这项研究表明,BTM保留了RNA质量,使得同一天的分子分析能够及时和精确地治疗患者.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 分子诊断学 分子诊断
- 生物标志物发现发现
背景情况:
- 及时诊断病对于有效治疗至关重要.
- 目前使用活检组织的方法需要大量的处理,阻碍了当天的诊断.
- 活检输送介质 (BTM) 是快速分子生物标志物评估的潜在来源.
研究的目的:
- 评估BTM作为病分子诊断的来源的实用性.
- 评估储存时间对BTM的RNA质量和产量的影响.
- 确定BTM是否可以提供与脏健康和疾病相关的转录信息.
主要方法:
- 优化RNA提取模仿BTM使用nephrectomy组织.
- 在存储时间从0.5小时到24小时后评估RNA产量和完整性.
- 使用qPCR,RNA测序和NanoString nCounter进行基因表达分析.
主要成果:
- 在BTM储存时间没有显著影响RNA质量或产量.
- 在BTM中的转录组概况在很大程度上与活检组织中的概况相似.
- 在BTM分析中,发现了与拒绝相关的基因表达特征,与班夫分数相关.
结论:
- BTM有效地保存了从脏活检中获得的转录信息.
- BTM具有显著的潜力,可以在同一天对脏疾病进行分子诊断.
- 使用BTM量身定制的qPCR面板可以实现快速,有针对性的病评估.
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