相关实验视频

Updated: Jan 31, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

以阿普塔默为媒介的有针对性的方法来破坏致癌性HSP90α

Jaskirat Kaur¹, Sakshi Nautiyal¹, Ipsita Roy¹

¹Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Sector 67, S.A.S. Nagar, Punjab, 160062, India.

International journal of biological macromolecules

|January 29, 2026

概括

新型RNA吸收酶选择性地向并抑制HSP90α,这是癌细胞增殖中至关重要的蛋白质. 这些胺体通过诱导亡和减少转移而表现出抗癌作用,而不会损害健康细胞.

科学领域:

生物化学生物化学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

细胞质热冲击蛋白90 (HSP90) 有两个主要异型:HSP90α和HSP90β.
在癌症和压力条件下,HSP90α显著上调,支持癌细胞增殖.
目前的HSP90抑制剂缺乏异型特异性,阻碍了临床应用.

研究的目的:

开发可选择性结合和抑制HSP90α的RNA体.
为了研究这些体的抗癌潜力.
评估异型特异性HSP90α抑制对治疗的影响.

主要方法:

从随机分类的寡核酸库中选择RNA体.
基于亲和性的结合试验,以比较对HSP90α和HSP90β的aptamer结合.
用于测量ATPase活性抑制的测试.
使用MCF7乳腺癌细胞进行细胞迁移测定.
西方斑点分析以评估客户端蛋白质表达.
在癌症和健康细胞上进行细胞活力测试.
亡测定. 亡测定. 死亡测定.

主要成果:

与HSP90β相比,精选的RNA吸收体对HSP90α的亲和力显著更高.
阿普塔默抑制了HSP90α ATPase的活性.
阿普塔默治疗降低了MCF7乳腺癌细胞迁移.
显著减少HSP90α特定客户端蛋白的表达.
亚胺类药物在癌细胞中诱导了显著的致死性,但在健康细胞中没有,通过亡.
细胞亡被证实是细胞死亡的机制.

结论:

可以专门选择RNA吸附体以准HSP90α.
这些胺酶抑制HSP90α功能,导致癌细胞的增殖和迁移减少.
亚胺体现出对癌细胞的选择性毒性,提供了潜在的治疗策略.
对传统的抗癌药物来说,阿普塔默是预防转移的有希望的替代品.

关键词:

癌细胞迁移癌细胞迁移癌细胞的增殖癌细胞的增殖在HSP90的异型中.蛋白质的特异性蛋白质的特异性它们是RNA吸附体.

更多相关视频

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

相关概念视频

Cancer-Critical Genes I: Proto-oncogenes

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...

Frustration and Conflict: Approach-Approach, Approach-Avoidance

Frustration and Conflict: Approach-Approach, Approach-Avoidance

Frustration occurs when people are obstructed or prevented from achieving a desired goal or fulfilling a perceived need. For example, when someone's input is ignored in a discussion, it can lead to feelings of frustration. Conflict, however, arises from opposing interests, goals, or actions. Conflicts can take various forms based on the nature of these opposing desires or goals.
One common type of conflict is the Approach–Approach Conflict. In this case, a person faces two desirable...

Nonsense-mediated mRNA Decay

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...

Actuarial Approach

Actuarial Approach

The actuarial approach, a statistical method originally developed for life insurance risk assessment, is widely used to calculate survival rates in clinical and population studies. This method accounts for participants lost to follow-up or those who die from causes unrelated to the study, ensuring a more accurate representation of survival probabilities.
Consider the example of a high-risk surgical procedure with significant early-stage mortality. A two-year clinical study is conducted,...