替代方法中的新方法方法 (NAMs):在脂肪干细胞球状体中对纳米结构酸的比较细胞毒性分析
Bianca Montenegro1,2, Rosângela Mayer Gonçalves1, Marcelle Gomes Pegurier1
1Laboratório de Biologia de Células Eucarióticas, Instituto Nacional de Metrologia, Qualidade e Tecnologia, Duque de Caxias, Rio de Janeiro, Brazil.
Journal of applied toxicology : JAT
|January 29, 2026
概括
与二维细胞培养相比,三维 (3D) 球体为纳米颗粒提供了更好的毒理风险评估. 这项研究证实了3D模型的存在.
科学领域:
- 纳米毒理学研究
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
背景情况:
- 传统的二维细胞培养在准确预测纳米材料毒理学风险方面存在局限性.
- 三维 (3D) 细胞模型,特别是球体,更好地模仿体内细胞环境和相互作用.
- 纳米粒子 (NP) 对医疗应用的批准需要使用先进模型进行强有力的毒理学评估.
研究的目的:
- 为了比较2D和3D球形系统中脂肪干细胞 (ASC) 的纳米毒理学评估.
- 在ASC球体中评估纳米结构碳化酸 (nCHA) 纳米颗粒的细胞毒性.
- 确定不同细胞毒性试验的适用性,以分析3D球状培养物.
主要方法:
- 在2D单层和3D球形中对ASC进行比较纳米毒理学分析,遵循经合组织的指导方针.
- 应用中性红色吸收和ATP量化试验来评估ASC球状体中的nCHA NP毒性.
- 确定二甲基硫酸盐 (SDS) 的IC50值,并分析处理后的球形形态.
主要成果:
- 在所有测试试验中,nCHA NPs在ASC单层或球体中没有表现出细胞毒性.
- 与2D培养相比,3D球体中SDS的细胞毒性显著更高,导致度依赖的分解.
- nCHA NPs没有改变ASC球状形态,并且被整合到细胞外基质中,模仿骨矿物质.
结论:
- 三维球体比二维培养更敏感,用于评估纳米粒子生物相容性和毒性.
- 3D球形模型代表了增强纳米毒理学评估的有希望的新方法方法 (NAMs).
- nCHA NP功能化的ASC球体显示出作为3D骨质生成模型和骨再生疗法的潜力.
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