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由短期季节性水环境压力相互作用驱动的ARG和VF的模式在一个优质高原湖泊中
Qing Li1,2, Jian Shen3,4,5, Jimeng Feng1,2,6
1Yunnan Dali Research Institute of Shanghai Jiao Tong University, Dali, 671000, China.
Microbial ecology
|January 29, 2026
概括
季节性变化会影响优化湖中的抗生素耐药性基因 (ARG) 和毒性因子 (VF). 雨季显示出更高的ARG多样性,而干季则由于环境压力导致了更大的VF丰度.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生态毒理学 生态毒理学
背景情况:
- 人类活动将抗生素,重金属和消毒剂引入水生生态系统.
- 这些污染物施加选择性压力,在微生物群落中促进抗生素耐药性基因 (ARG) 和毒性因子 (VF).
- 以前对高原湖中的ARG和VF的研究是有限的,特别是关于季节变化和环境影响的研究.
研究的目的:
- 在不同季节的高原湖Qilu湖中调查ARG和VF的多样性和丰富性.
- 分析水文和水质参数之间的关系以及ARG和VF的流行情况.
- 了解微生物机制驱动的耐药性和毒性,以应对环境变化.
主要方法:
- 季节性采样Qilu湖的水.
- 分析水文数据 (输入) 和水质参数 (有机物,金属离子,总,pH).
- 超基因组分析用于识别和量化ARG和VF.
- 相关性分析以确定环境因素,ARG和VF之间的关系.
主要成果:
- 在干旱和潮湿季节之间观察到水文和水质的显著差异.
- 与干旱季相比,在雨季记录的ARG和VF的多样性和丰度更高.
- 糖类ARG是最丰富的;LPS和IV型 pili分别是干旱和潮湿季节的主导VF.
- Pseudomonadota 在两个季节中显示了ARG和VF的最高丰度.
- 观察到ARG和VF之间的正相关性,在干旱季节更强.
结论:
- 季节性变化,特别是水文变化和水质变化,显著影响了优质高原湖中的ARG和VF动态.
- 在雨季增加的ARG多样性与外源性细菌输入和增强的水平基因转移有关.
- 干旱季的条件,包括低水量和金属离子应力,提高了核心VF的相对丰度,导致总体VF度更高.
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