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Updated: Jan 31, 2026

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通过在塞尔托利细胞中的mTORC2-PKCα通路促进细胞骨组织,PTBP1支持小鼠精子生成
Manabu Ozawa1,2, Jumpei Taguchi3, Hideto Mori4
1Core Laboratory for Developing Advanced Animal Models, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan. semil@ims.u-tokyo.ac.jp.
Communications biology
|January 29, 2026
概括
聚胺系结合蛋白1 (PTBP1) 对于男性生育能力至关重要. 它通过通过mTORC2信号调节细胞骨来维持塞尔托利细胞结构和精子生产.
科学领域:
- 生殖生物学 生殖生物学
- 分子细胞生物学 分子细胞生物学
- 精子生成规范 精子生成规范
背景情况:
- 塞尔托利细胞对于精子生成至关重要,需要完整的细胞骨才能发挥作用.
- 控制塞尔托利细胞细胞骨动态的调控机制尚未完全理解.
研究的目的:
- 研究聚胺管结合蛋白1 (PTBP1) 在塞尔托利细胞功能和男性生育能力中的作用.
- 阐明在塞尔托利细胞中由PTBP1调节的分子通路.
主要方法:
- 在小鼠中,Sertoli 细胞特异的 Ptbp1 缺失.
- 纯化的塞尔托利细胞的转录基因分析.
- RNA免疫沉降序列 (RIP-seq) 的测序.
- 对细胞骨完整性和F-actin的评估.
- 使用构成性活性PKCα的功能性救援实验.
主要成果:
- 塞尔托利细胞特异性Ptbp1删除导致生殖细胞丧失,血液丸屏障破坏和男性不孕症.
- 删除PTBP1导致细胞骨和粘附相关基因的失调.
- PTBP1直接与Rictor mRNA结合,减少RICTOR蛋白和PKCα的激活.
- 观察到F-actin细胞骨和Sertoli细胞结构受损.
- 恢复PKCα活动挽救了细胞骨缺陷.
结论:
- PTBP1对于维持塞尔托利细胞结构和精子生成至关重要.
- 通过mTORC2-PKCα信号通路,PTBP1充当男性生育能力的转录后调节者.
- PTBP1维持了塞尔托利细胞中的细胞骨完整性,这对男性生殖功能至关重要.
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