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在乳牛中诱导肠道屏障功能障碍:评估新的血清炎症标志物和量化肠道超透性的方法
K E Vagnoni1, E Lopez-Cruz1, M Carranza1
1Department of Animal Science, California Polytechnic State University, San Luis Obispo, CA 93407.
阿司匹林诱导小牛的肠壁功能障碍,增加了FABP2和TNF血清标志物. 泽西公牛表现出比霍尔斯坦更高的肠道透性,这表明FABP2和TNF是肠道健康的潜在标志物.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 动物科学动物科学
- 胃肠病学 胃肠病学
背景情况:
- 肠道屏障功能障碍,包括炎症和超透性,可能会对奶牛的生产力产生负面影响.
- 众所周知,农场中的压力因素,如小酸性和热应激,会损害肠道健康.
- 研究肠道屏障功能对于改善动物健康和生产力至关重要.
研究的目的:
- 在试验中诱导小牛的肠道屏障功能障碍.
- 评估肠道健康的新型血清炎症标志物.
- 评估一种用于测量肠道超透性的新方法.
主要方法:
- 口服阿司匹林 (200毫克/公斤体重/天) 给荷尔斯坦和泽西公牛21天.
- 使用口服Cobalt-EDTA (Co-EDTA) 剂量和尿液Co:创造氨酸比率来测量肠道透性.
- 对血清炎症标志物的分析:哈普托格洛宾 (Hp),脂多糖结合蛋白 (LBP),脂肪酸结合蛋白2 (FABP2) 和瘤缩因子 (TNF).
主要成果:
- 服用阿司匹林会增加血清FABP2和TNF度.
- 与荷尔斯泰因相比,泽西公牛的尿液Co:肌素比率表明,与荷尔斯泰因相比,泽西公牛的肠道透性更高.
- 血清TNF度与尿路Co分泌有显著的相关性,Co是肠道透性的标志物.
- 血清Hp和LBP水平不受阿司匹林治疗的影响.
结论:
- 血清FABP2和TNF显示出作为评估牛肠道屏障功能障碍的有价值生物标志物的潜力.
- 在荷尔斯坦和泽西公牛之间观察到肠道透性的品种差异.
- 联合EDTA方法提供了一种可靠的方法来测量肠道的超透性.
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