功能化链接化学对细胞酶固定化的影响:对TEOS,APTS和MPTS功能化磁纳米粒子进行比较研究
Larissa Lamburghini Brandão1,2, Thais de Andrade Silva1,2, Ana Carolina de Lima Barizão1
1Center for Research, Innovation and Development of Espírito Santo - CPID Espírito Santo Brazil jairo.oliveira@ufes.br.
RSC advances
|January 30, 2026
概括
用 (3-mercaptopropyl) trimethoxysilane (MPTS) 功能化的磁性纳米生物催化剂显示出卓越的稳定性和可用于细胞酶固定的可重复使用性. 在纳米生物催化剂设计中,连接物化学是平衡催化效率和长期性能的关键.
科学领域:
- 生物技术和纳米材料科学 生物技术和纳米材料科学
- 酶工程和固定化工程
背景情况:
- 细胞酶固定对于高效的酶过程至关重要.
- 磁纳米粒子为生物催化剂的回收和再利用提供了优势.
- 纳米颗粒的表面功能化影响酶负载,活性和稳定性.
研究的目的:
- 开发和评估用于细胞酶固定化的磁性纳米生物催化剂.
- 研究不同表面功能化连接体 (APTS与MPTS) 对纳米生物催化剂性能的影响.
- 为设计稳定和高效的磁性纳米生物催化剂提供准则.
主要方法:
- 用TEOS涂层的Fe3O4纳米颗粒的合成,并使用APTS或MPTS进行功能化.
- 纳米生物催化剂的物理化学表征.
- 酶固定能力和动力学研究.
- 在不同的条件下 (pH,温度) 进行酶活性测定.
- 运行稳定性和可重复使用性测试.
- 分子对接模拟. 分子对接模拟.
主要成果:
- MNPs@TEOS-MPTS表现出更高的固定能力 (1.06 mg/mg) 并遵循伪二次动力学.
- MNPs@TEOS-APTS显示较低的负载 (0.94 mg/mg) 与扩散有限的行为.
- MNPs@TEOS-APTS表现出较高的初始催化活性 (1.92 U/g),但性pH耐受性较差.
- MNPs@TEOS-MPTS表现出较低的初始活性 (1.61 U/g),但具有卓越的耐热性和运行稳定性.
- 在六个周期后,MNP@TEOS-MPTS保持了62%的活动,超过了MNP@TEOS-APTS (33%).
- 分子对接表示MPTS促进形状刚性和稳定性.
结论:
- 连接体的化学作用极大地影响了磁性纳米生物催化剂的性能.
- MPTS的功能化增强了细胞酶加载纳米生物催化剂的操作稳定性和可重复使用性.
- APTS的功能化有利于更高的初始催化活性,但损害了稳定性.
- 调整连接物选择对于优化纳米生物催化剂用于特定的工业应用至关重要.
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