在子宫内膜癌中,COL23A1通过METTL14-m6A-YTHDF1轴促进瘤的进展和糖溶性重编程
Huazhen Wu1, Miao Ke2, Xiaoli Feng1
1Department of Radiotherapy, The Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China China.
Cytotechnology
|January 30, 2026
概括
原体XXIII型α1 (COL23A1) 通过重编程细胞代谢,驱动子宫内膜癌的进展. 针对METTL14-m6A-YTHDF1-COL23A1通路为这种恶性瘤提供了潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 子宫内膜癌 (EC) 的进展涉及细胞外基质重塑和代谢重编程.
- 在EC中,XXIII型α1原体 (COL23A1) 的作用在很大程度上仍未被描述.
研究的目的:
- 研究子宫内膜癌中COL23A1的表达,功能和调节机制.
- 阐明METTL14-m6A-YTHDF1-COL23A1轴在EC进展和代谢中的作用.
主要方法:
- 分析公开数据集 (TCGA,GTEx,cBioPortal) 的 COL23A1 表达和相关性.
- 在体外测定 (qRT-PCR,西部斑点,CCK-8,殖民地形成,流细胞计,Transwell,海马) 来评估COL23A1功能.
- 代谢学,RNA免疫沉降 (RIP) 和异种移植模型用于研究调节机制和体内效应.
主要成果:
- COL23A1在EC上升调节,与高级特征和低生存率相关.
- COL23A1沉默抑制了EC细胞的增殖,迁移,入侵和瘤生长.
- COL23A1的倒置破坏了糖解,减少了ECAR,增加了OCR.
- METTL14和YTHDF1通过m6A修饰来调节COL23A1的表达,从而促进瘤原体的表型.
结论:
- COL23A1作为子宫内膜癌的致癌驱动因素,促进进展和依赖于糖溶解的代谢重编程.
- 对于EC细胞表型来说,METTL14-m6A-YTHDF1-COL23A1轴非常重要.
- 准这一轴为子宫内膜癌提供了潜在的治疗策略.
相关概念视频
Tumor Progression
7.4K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.4K
mTOR Signaling and Cancer Progression
4.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.8K
Other Glycolytic Pathways
903
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
903
Hypothalamic-Pituitary Axis
66.0K
The response to stress—be it physical or psychological, acute or chronic—involves activation of the Hypothalamic-Pituitary-Adrenal (HPA) axis. The HPA axis is part of the neuroendocrine system because it involves both neuronal and hormonal communication. Its function is to regulate homeostatic systems—metabolic, cardiovascular, and immune—providing the necessary means to respond to a stressor.
66.0K
The Eukaryotic Promoter Region
18.8K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.8K
Cancer Stem Cells and Tumor Maintenance
6.0K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.0K


