由Helicobacter pyloriCagA通过IKKββ的Src-cortactin依赖型氨酸酸化来增强ADP-肝素依赖的NF-κB激活
Irshad Sharafutdinov1, Nicole Tegtmeyer1, Barbara Friedrich1
1Department of Biology, Division of Microbiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany.
microLife
|January 30, 2026
概括
杆菌通过CagA-cortactin-FAK-Src-IKKβ通路激活NF-κB和IL-8的分泌. 这项研究揭示了一种新的信号级联,有助于H. pylori诱导的炎症.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 微生物学 微生物学
背景情况:
- 激活B细胞的核因子卡帕-光链增强剂 (NF-κB) 对先天免疫非常重要.
- 杆菌利用皮质素进行介质素-8 (IL-8) 的分泌,这取决于NF-κB的激活.
- 在 H. pylori 诱导的 IL-8 释放中,皮质素的精确信号传递机制尚不清楚.
研究的目的:
- 阐明涉及H. pylori介导的NF-κB激活和IL-8分泌的特定皮质素信号通路.
- 为了研究皮质素,CagA,FAK,Src和TIFA在H. pylori感染期间NF-κB级联中的作用.
主要方法:
- 利用皮质素,FAK和TIFA淘汰细胞来评估NF-κB通路的激活.
- 感染了H. pylori的细胞,并用纯化的腺二酸盐 (ADP) -β-d-曼诺-肝素 (ADPH) 和CagA.
- 测量了IKKβ和p65NF-κB的酸化,以及IL-8的分泌.
主要成果:
- 皮质素淘汰细胞显示FAK和Src激活减少,减少IKKβ和p65酸化.
- 在野生类型细胞中,ADPH刺激的NF-κB激活和IL-8分泌被CagA增强.
- 在野生类型的细胞中观察到ADPH和CagA的联合作用,但在淘汰细胞中没有,突出显示了协同作用.
结论:
- 在H. pylori诱导的炎症反应中发现了一种新的CagA>皮质动脉>FAK>Src>IKKβ信号级联.
- 这一途径对于ADPH依赖的NF-κB激活和IL-8分泌至关重要.
- 通过这种新发现的级联,CagA增强了H. pylori介导的炎症信号传递.
关键词:
这是一种ADP-heptose.这就是CagAA.这是假的,假的,假的.在Helicobacter Pylori的研究中,在 NF-κBB 中.美国Src Src这就是T4SS.这就是TIFA TIFA.皮质组织蛋白 (cortactin) 是一种皮质组织蛋白.这是一种炎症炎症炎症炎症.介质蛋白-8-8的使用方法更多相关视频
11:44A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
Published on: January 24, 2016
12.5K
10:44One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Published on: June 18, 2016
10.1K
相关概念视频
02:26NF-κB-dependent Signaling Pathway
10.0K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
10.0K
01:21Frequency-dependent Selection
23.9K
When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
23.9K
01:17Drug Dependence
1.7K
Medications are typically administered to achieve therapeutic effects. Some drugs can modify an individual's mood and perception, frequently resulting in various enjoyable experiences. However, this can result in drug dependency, a condition marked by continuous drug use despite potential negative consequences. Drug dependency primarily falls into two categories: psychological and physical dependence. Psychological dependence occurs when the pleasurable feelings induced by the drug...
1.7K
02:55Temperature Dependence on Reaction Rate
89.0K
The Collision Theory
Atoms, molecules, or ions must collide before they can react with each other. Atoms must be close together to form chemical bonds. This premise is the basis for a theory that explains many observations regarding chemical kinetics, including factors affecting reaction rates.
The collision theory is based on the postulates that (i) the reaction rate is proportional to the rate of reactant collisions, (ii) the reacting species collide in an orientation allowing contact between...
Atoms, molecules, or ions must collide before they can react with each other. Atoms must be close together to form chemical bonds. This premise is the basis for a theory that explains many observations regarding chemical kinetics, including factors affecting reaction rates.
The collision theory is based on the postulates that (i) the reaction rate is proportional to the rate of reactant collisions, (ii) the reacting species collide in an orientation allowing contact between...
89.0K
01:19Contact-dependent Signaling
47.0K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
Gap Junctions
In animal cells, gap junctions are formed...
47.0K
01:16Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
1.3K
Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
1.3K