一个Caspase-3可激活的近红外AIEgen用于瘤亡成像在vivo中
Lingling Xu1,2, Yuanyuan Jin2, Zhanjun Yang2
1Breast Surgery, Wenzhou Central Hospital, Wenzhou 325000, China.
Chemical & biomedical imaging
|January 30, 2026
概括
研究人员开发了一种新型的近红外 (NIR) 聚合诱导发射光原体 (AIEgen),通过成像瘤中的caspase-3活性来追踪抗癌药物的有效性. 这种新的AIEgen可以在体内对治疗反应进行敏感的实时监测.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 纳米技术纳米技术
- 化学生物学 化学生物学
背景情况:
- 近红外 (NIR) 光成像允许通过检测瘤caspase-3活性来实时监测抗癌药物的疗效.
- 聚合诱导排放光原体 (AIEgens) 是敏感的光体,但用于成像caspase-3活性的NIR AIEgens缺乏.
研究的目的:
- 开发一种可激活的NIR AIEgen,用于敏感的成像,用于在细胞和瘤中检测caspase-3活性.
- 评估开发的AIEgen在实时监测抗癌药物治疗效果方面的潜力.
主要方法:
- 一个可激活的NIR AIEgen,Ac-Asp-Glu-Val-Asp-Pra-QMT (Ac-DEVD-Pra-QMT) 的合成.
- 在体外和体内实验中评估卡斯巴-3活性成像在西斯普拉丁诱导的细胞和瘤中.
- 测量NIR光强度在caspase-3裂变和聚合时的变化.
主要成果:
- Ac-DEVD-Pra-QMT已成功开发为可激活的NIR AIE基因.
- 在形细胞中发生的caspase-3裂变时,Ac-DEVD-Pra-QMT形成纳米粒子,激活NIR光.
- 与对照组相比,NIR光强度在体外增加了14.9倍,在体内增加了2.7倍.
结论:
- 在apoptotic细胞和瘤中,Ac-DEVD-Pra-QMT有效地描绘了caspase-3活动.
- 开发的NIR AIEgen显示出对化学疗法效应的敏感实时跟踪具有重大潜力.
- 这种工具可以帮助在癌症治疗中早期监测治疗反应.
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