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毛囊液细胞外囊泡通过脂质和线粒体调节改善牛卵细胞质量
Fernanda Schneberger1, Alessandra Bridi1, Priscila Assis Ferraz1
1Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, Brazil.
Frontiers in veterinary science
|January 30, 2026
概括
毛囊液细胞外囊泡 (ffEVs) 通过改善线粒体活性和脂质代谢来提高体外成熟期间的牛卵细胞质量,从而在辅助生殖技术中提高了胚胎细胞质量. 这项研究强调了ffEVs作为改善胚胎生产的有希望的补充剂.
科学领域:
- 生殖生物学 生殖生物学
- 细胞的新陈代谢
- 细胞外囊泡 细胞外囊泡
背景情况:
- 试管成熟 (IVM) 系统难以复制卵泡液 (FF) 微环境,影响卵细胞细胞质能力和胚胎质量.
- 在FF内的细胞外囊泡 (EVs) 对于细胞间通信至关重要,但在IVM系统中往往不存在.
研究的目的:
- 研究FFEVs对牛卵细胞成熟和质量的影响.
- 评估 ffEVs 对卵细胞和随后的胚胎细胞代谢参数和整体质量的影响.
主要方法:
- 卵细胞接受了带有或没有ffEV的IVM,随后对核成熟,线粒体活性,活性氧物种 (ROS) 和脂质代谢进行了评估.
- 评估了胚胎发育率和胚胎质量 (细胞数量,线粒体活性,脂滴面积).
- 与脂质代谢和抗氧化酶相关的mRNA和miRNA表达在卵细胞,累积细胞 (CC) 和ffEV中进行了分析.
主要成果:
- ffEVs提高了卵细胞的细胞质能力,增加了线粒体活动,降低了ROS水平,但没有影响核成熟.
- 卵细胞脂质滴滴面积随着ffEVs的增加而增加,这表明增强了脂质发生或脂质吸收.
- 胚胎细胞的线粒体活性和脂质液滴面积下降,表明对胚胎质量有益的代谢调节.
结论:
- ffEVs积极改变卵细胞代谢,提高卵细胞质量和胚胎细胞脂质含量.
- ffEV显示出作为一种添加剂的潜力,可以改善牛胚胎体外生产 (IVP) 系统中的IVM条件.
- 虽然ffEVs含有调节分子,但它们在IVM后对卵细胞和CC基因表达的直接影响是有限的,除了CC中的bta-miR-23b-3p下调外.
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