两种MALDI-TOF微生物识别MS系统的比较评估:临床微生物学实验室的准确性和工作流程效率
Jasmin Köffer1, Jale Ören1, Ulrike Betz1
1MVZ Laboratory Dr. Limbach, Heidelberg, Germany.
Microbiology spectrum
|January 30, 2026
概括
维特克MS PRIME (VMP) 和Maldi Biotyper Sirius (BS) 系统显示了微生物物种的高识别精度. 与BS相比,VMP提供了更有效的工作流程,更短的动手时间和更快的结果.
科学领域:
- 微生物学 微生物学
- 分析化学 分析化学
- 临床实验室科学 临床实验室科学
背景情况:
- 在临床实验室快速识别微生物时,矩阵辅助激光脱/离子化飞行时间质谱 (MALDI-TOF MS) 非常重要.
- 对比不同的MALDI-TOFMS系统对于优化实验室工作流程和诊断准确性至关重要.
研究的目的:
- 在常规实验室环境中比较VITEK MS PRIME (VMP) 和MALDI Biotyper Sirius (BS) 系统的识别准确度和工作流效率.
- 评估两种系统的性能,使用大量多样化的微生物分离物,包括罕见物种和阳性血液培养物.
- 评估短化时间和酸处理对鉴定结果的影响.
主要方法:
- 使用VMP和BS系统分析了代表219个物种的927个分离物.
- 在各种短期潜伏条件下 (4-6小时) 测试了119个阳性血液培养物,有或没有酸.
- 实践时间 (HOT) 和结果时间 (TTR) 的测量,模拟例行实验室工作负载.
主要成果:
- 无论是VMP还是BS,都显示出高的整体识别率 (VMP的95.5%,BS的91.1%).
- 与BS (88.2%) 相比,VMP在短化血液培养中表现优异 (99.2%没有甲状酸).
- VMP工作流分析表明,较短的HOT (106分钟) 和TTR (191分钟) 与BS相比,成功识别率 (98%) 高 (155分钟的HOT/TTR,成功率为82%).
结论:
- 两种MALDI-TOF MS系统都表现出了很好的一致性,用于识别广泛的物种.
- 维特克MS PRIME系统提供了更精简的工作流程,提高了效率和准确性,特别是对于具有挑战性的样品,如阳性血液培养等.
- 选择VMP和BS取决于实验室的具体需求,但两者都可以提高实验室的效率.
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