芯片上的实验室用于在工业固态发酵过程中监测酶活性,这与R2R制造兼容
Verónica Mora-Sanz1, Alvaro Conde2, Elisabeth Hengge3
1TECNALIA, Basque Research and Technology Alliance (BRTA), Mikeletegi Pasealekua 2, 20009 Donostia-San Sebastian, Spain. veronica.mora@tecnalia.com.
Lab on a chip
|January 30, 2026
概括
一个新的一次性实验室芯片 (LoC) 能够快速,经济高效地对固态发酵 (SSF) 的色度测量酶活性进行监测. 这项技术可以快速检测诸如α-氨酶和细胞酶等关键酶,这些酶对工业过程至关重要.
科学领域:
- 生物技术是生物技术.
- 微流体学 微流体学
- 分析化学 分析化学
背景情况:
- 固态发酵 (SSF) 工艺对于工业应用至关重要,包括生物剂生产.
- 在SSF期间监测酶活性对于过程优化和质量控制至关重要.
- 现有的酶活性监测方法可能耗时且昂贵.
研究的目的:
- 开发一种一次性芯片上的实验室 (LoC),用于在SSF中高效地测色酶活性监测.
- 将酶基质直接集成到芯片上,以提高用户友好性和稳定性.
- 使用智能手机集成创建一个便携式系统来实时检测酶活动.
主要方法:
- 使用具有成本效益的卷对卷 (R2R) 挤出涂层制造微流体芯片.
- 在微流体芯片中利用毛细管驱动的流量进行自我填充和精确的液体处理.
- 干燥的酶基质 (α-氨酶和细胞酶) 通过滴/冷干燥或压电沉积/空气干燥进行整合.
- 开发一个口袋尺寸的色度计阅读器和一个用于数据采集和分析的Android应用程序.
主要成果:
- 开发的LoC系统在10分钟内成功检测到SSF样本中的α-氨酶和细胞酶活性.
- 使用商业酶的验证确定了检测极限 (LoD).
- 优化的储存条件提高了试剂的保质期和稳定性.
- 该系统与工业工厂的样本证明了功能,显示在发酵过程中酶活性增加.
结论:
- 一次性LoC提供了一个快速,用户友好和经济有效的解决方案,用于监测SSF中的关键酶活动.
- 集成的干式试剂格式和与智能手机兼容的阅读器增强了该系统在工业应用中的实用性.
- 这项技术有助于实时监控和优化生物洗剂生产和其他基于SSF的行业的过程.
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